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Caspase Activity Assays & Active Enzymes

Caspase-1 Assay Kit for Drug Discovery

  • Flexible format allows user to choose between a colorimetric substrate or fluorogenic substrate
  • Can be used to screen inhibitors of caspase-1, study enzyme regulation and kinetics, or cleave target substrates
 

Inhibition of Caspase-1 by Ac-YVAD-CHO



The enzyme was incubated with the inhibitor for 10 minutes prior to addition of substrate. 30 U/well; [YVAD-CHO]=0.1 mM; [YVAD-pNA]=200 mM; 25° C

Kinetics of YVAD-pNA cleavage by Caspase-1



30 U/well; 25°C. Rates were obtained from the slopes of the initial, linear portion of plots of A405 vs. time. Curve and kinetic parameters derive from a non-linear least squares fit to the Michaelis-Menten equation (Marquadt algorithm).

 






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For Research Use Only. Not for use in diagnostic procedures.
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