Inhibition of Caspase-1 by Ac-YVAD-CHO
The enzyme was incubated with the inhibitor for 10 minutes prior to addition of substrate. 30 U/well; [YVAD-CHO]=0.1 mM; [YVAD-pNA]=200 mM; 25° C
Kinetics of YVAD-pNA cleavage by Caspase-1
30 U/well; 25°C. Rates were obtained from the slopes of the initial, linear portion of plots of A405 vs. time. Curve and kinetic parameters derive from a non-linear least squares fit to the Michaelis-Menten equation (Marquadt algorithm).
Caspase-3 cell assay kit |
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| Colorimetric detection, Fluorescent detection, HTS, Activity assay | Print as PDF | ||||||
| BML-AK703-0001 | 96 wells | 531.00 USD |  |
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Caspase-1 (human), (recombinant) |
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| Highly active caspase essential for apoptosis. | ||||||
| Produced in E. coli. cDNA encodes residues identical to Asn120-His404 (C-terminus) at Genbank Accession No. M87507, except for an Asp381 to Glu change, introduced to stabilize the enzyme against autoproteolysis., ≥90% (SDS-PAGE) | Print as PDF | ||||||
| BML-SE168-5000 | 5000 U | 554.00 USD | |
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Caspase-3 (human), (recombinant) |
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| Produced in E. coli. Caspase-3 (CPP32; Yama; apopain) from human cDNA. The enzyme was cleaved and activated from the proenzyme, ≥95% (SDS-PAGE) | Print as PDF | ||||||
| BML-SE169-5000 | 5000 U | 531.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Caspase-4 (human), (recombinant) |
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| Produced in E. coli., ≥90% (SDS-PAGE) | Print as PDF | ||||||
| BML-SE176-5000 | 5000 U | 531.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Caspase-5 (human), (recombinant) |
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| Produced in E. coli, ≥80% (SDS-PAGE) | Print as PDF | ||||||
| BML-SE171-5000 | 5000 U | 531.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
Caspase-6 (human), (recombinant) |
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| Highly, active caspase-6 for the study of enzyme kinetics or screening of inhibitors | ||||||
| Produced in E. coli., ≥80% (SDS-PAGE) | Print as PDF | ||||||
| BML-SE170-5000 | 5000 U | 528.00 USD | |
|||
| Do you need bulk/larger quantities? | ||||||
Caspase-8 (human), (recombinant) |
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| Highly active caspase-8 for the study of enzyme regulation and kinetics | ||||||
| Produced in E. coli, ≥90% (SDS-PAGE) | Print as PDF | ||||||
| BML-SE172-5000 | 5000 U | 531.00 USD | |
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| Do you need bulk/larger quantities? | ||||||
