Recognizes procaspase-8 and active caspase-8. Detects procaspase-8 (p55/54), the intermediate cleavage products (p43/41) and the p18 active subunit of caspase-8 by Western blot
Does not cross-react with mouse caspase-8
Validated for Western blot, Immunoprecipation and Immunohistochemistry applications
(A) Schematic structure of caspase-8 and its cleavage products as detected by anti-caspase-8 (12F5). The antibody is directed against the p18 subunit and detects procaspase-8 (p55/54), the intermediate cleavage products of 43kD and 41kD and the p18 active subunit of caspase-8.
(B) Detection of Fas ligand-induced caspase-8 processing and activation in human Jurkat cells.