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Comprehensive Solutions for Apoptosis

Monitor programmed cell death from membrane to nucleus


  • Membrane Display Of Phosphatidylserine
  • Chromatin Condenstion
  • DNA Damage

  • Caspase Activity Assays & Active Enzymes
  • Ready-to-Use Caspase Inhibitors
  • Antibody-Based Detection of Cleaved Caspases

  • Death Receptor Ligands

  • High-Throughtput Assays for Mitochondrial Function

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Apoptosis Brochure

Complete Analysis of Apoptosis and Cell Fate

Intrinsic. Extrinsic. Simplistic.

Of the three major established programmed cell death (PCD) pathways, Apoptosis (PCD Type 1) is the most-well characterized, being recognized as a critical regulator of development, immunity, as well as organ and tissue homeostasis. Apoptotic cells die in a controlled fashion in response to a variety of extrinsic or intrinsic signals (e.g., activation of TNF receptors, DNA damage, mitochondrial pathways). Influencing cells to undergo or resist apoptosis is the aim of countless drugs (both in use and development) for treatment of cancer, neurodegenerative, and immune-related disease. The interplay between apoptotic, autophagic, and necrotic pathways promises to yield further targets for therapeutic intervention. With over 2000 products for the analysis of cell death, Enzo enables detection of phenotypic hallmarks of apoptotic cell death (see figure below), and the complete analysis of cross-talk between PCD pathways.

 

Apoptotic Pathways (PCD Type 1)

Apoptosis Detection Guide

Find your apoptosis detection solution with our simple guide

Enzo has a broad range of solutions that enable the investigation of membrane changes, DNA modifications, mitochondrial changes and more.

Find the appropriate assay for your application. Start by picking your sample type and let this tool guide you to identify the appropriate assay.


 

Detect Hallmarks Of Apoptosis

Cells undergoing apoptosis display morphological and molecular features such as nuclear condensation, DNA fragmentation, membrane “blebbing” and phagocytosis by another cell. Enzo’s expertise in fluorescent probe chemistry and cellular analysis combine to provide high-sensitivity fluorescent probes for characterizing cell membrane and organelle changes associated with apoptosis.

 

Membrane Display Of Phosphatidylserine

Real-time, Multiplex Analysis of Phosphatidylserine Exposure and Membrane Permeability

Detect Cell Death in GFP-Expressing Cell Lines

Chromatin Condensation

Sensitive, High-throughput Detection of Chromatin Condensation


Convenient Alternative to Hoechst dyes that require 350nm UV laser for detection

DNA Damage

Vizualize DNA Fragmentation in Apoptotic Cells

Comet SCGE Assay Kit

Modulate & Screen Caspase Activity

Activation of apoptotic caspases can lead to inactivation or activation of substrates, and the initiation of a signaling cascade resulting in the controlled demolition of cellular components. Our collection includes convenient caspase activity assays containing high-purity, high-activity enzymes and cell-permeable peptide substrates and inhibitors for research and drug discovery applications.

 

Caspase Activity Assays & Active Enzymes

Real-time, Multiplex Analysis of Phosphatidylserine Exposure and Membrane Permeability


Inhibition of Caspase-1 by Ac-YVAD-CHO
The enzyme was incubated with the inhibitor for 10 minutes prior to addition of substrate. 30 U/well; [YVAD-CHO]=0.1 mM; [YVAD-pNA]=200 mM; 25° C

Ready-to-Use Caspase Inhibitors

High purity, peptide inhibitors for modulation of the caspase signaling cascade.


Antibody-Based Detection of Cleaved Caspases

Wide range of mabs and pabs against caspases


 

Extrinsic Signaling Through Death Receptors

TNF ligands and receptors act to regulate cell responses including activation, proliferation, differentiation, and apoptosis. Experience the purity, sensitivity, and reproducibility of Enzo’s carefully, engineering and optimized TNF Superfamily Products.

 

Death Receptor Ligands

Members of the tumor necrosis factor (TNF) receptor gene superfamily bind to extrinsic ligands and transduce intracellular signals leading to the destruction of the cell. Ligands, such as FasL and TNF-alpha, hold potential as targets for therapeutic and diagnostic applications.




Oligomerization Domains in Enzo Enhanced Ligands:
MEGA™Ligands – use the ACRP30headless domain from human or mouse ACRP30
KILLER™Ligands – use a proprietary linker peptide that promotes trimerization
SUPERKILLER™Ligands – use the KILLER™ linker peptide mutated to increase disulfide-mediated cross-linking

Featured Product

SUPERFASLIGAND™ (soluble) (human), (recombinant)

  • Increased stability
  • Enhanced immune activation compared to other recombinant ligands
  • Mimics glycosylation of native human FasL
 

Mitochondrial Perturbations & Intrinsic Activation

The collapse of mitochondrial membrane potential (MMP) coincides with the opening of the mitochondrial permeability transition pores, release of cytochrome C into the cytosol, and triggers additional downstream events in the apoptotic cascade. Enzo’s MITO-ID® reagents and kits are optimal for use in demanding cell analysis applications involving mitochondrial perturbations.

 

High-Throughtput Assays for Mitochondrial Function

A real-time mitochondrial membrane potential assay with superior sensitivity

  • More sensitive than JC-1
  • True mix-and-read homogeneous assay for live cells
  • Validated for multiple detection platforms: microscopy, flow cytometry and microplates

Dual-emission Dye Monitors Energetic Status

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For Research Use Only. Not for use in diagnostic procedures.
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