MMP RED and GREEN Drug Discovery Kits

• Improved red-shifted substrate with better kinetics and brighter signal, allowing for lower substrate concentrations and resulting in less compound interference
• Includes active recombinant enzyme, substrate, and assay buffer
• Convenient real-time kinetics of cleavage is easily determined
• Microplate format for high-throughput screening
• Detailed instructions provided

The OmniMMP^TM RED and MMP-3 Fluorogenic Substrates, provided in the kits, offer key advantages over other MMP substrates.

1. Emission at the higher end of the spectrum (576nm and 521nm, respectively, after excitation at 545nm and 494nm) avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium.
2. Highly quenched (very low background), but once cleaved by MMPs emits extremely bright signal.
3. MMP substrate peptides inherently display poor aqueous solubility, often with K_ms near their limits of solubility, making enzyme and inhibitor kinetics difficult. MMP K_ms for OmniMMP^TM RED and MMP-3 Fluorogenic Substrates are well below the solubility limits of the substrates.
4. In addition to the efficient binding as exhibited by low Kms, OmniMMP^TM RED and MMP-3 Fluorogenic Substrates are avidly cleaved by MMPs, with k_cat/K_ms in the range of 10⁵-10⁷ M^-1sec^-1.
5. Better kinetics allows lower substrate concentrations, avoiding inhibition by the substrate or competition with the inhibitor at the active site.
6. The ultra-strong fluorescence of OmniMMP^TM RED and MMP-3 Fluorogenic Substrates allows for substrate concentrations much lower than the K_m, a condition generally desirable in inhibitor screening assays.