Replaces Prod. #: BML-AP401
Propidium iodide (PI) belongs to the same chemical class as ethidium bromide. As with ethidium bromide, its fluorescence is enhanced 20-30-fold upon binding to nucleic acids. The fluorescence excitation maximum is red-shifted 30-40 nm and the fluorescence emission maximum blueshifted ~15 nm. PI also binds to RNA as does DAPI and acridine orange. PI is membrane impermeant and is commonly used for identifying dead cells in a mixed population of cells and as a counter-stain in multicolor fluorescent techniques. It can also be used to differentiate necrotic, apoptotic and viable cells. The dye is suitable for fluorescence microscopy, flow cytometry and fluorometry applications. Wavelength Maxima: Excitation 535nm, Emission 617nm
Product Details
| Formula: | C27H34I2N4 |
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| MW: | 668.4 |
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| CAS: | 25535-16-4 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Dark red solid. |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Fundamental Role of Pentose Phosphate Pathway within the Endoplasmic Reticulum in Glutamine Addiction of Triple-Negative Breast Cancer Cells: C. Marini, et al.; Antioxidants
12, 43 (2023),
Abstract;
Mitochondrial Generated Redox Stress Differently Affects the Endoplasmic Reticulum of Circulating Lymphocytes and Monocytes in Treatment-Naïve Hodgkin’s Lymphoma: C. Marini, et al.; Antioxidants
11, 762 (2022),
Abstract;
NLRP3 Inflammasome Is Activated in Rat Pancreatic Islets by Transplantation and Hypoxia: V. Lavallard, et al.; Sci. Rep.
10, 7011 (2020),
Abstract;
Full Text
Omega-3 docosahexaenoic acid induces pyroptosis cell death in triple-negative breast cancer cells: N. Pizato, et al.; Sci. Rep.
8, 1952 (2018),
Abstract;
The novel protective role of P27 in MLN4924-treated gastric cancer cells: Q. Zhang, et al.; Cell. Death Dis.
6, e1867 (2015),
Application(s): Viability staining,
Abstract;
Full Text
High-throughput cytotoxicity screening by propidium iodide staining: B.S. Edwards, et al.; Curr. Protoc. Cytom.
Chapter 9, Unit9 24 (2007),
Abstract;
