Predictive toxicology is a pivotal aspect of modern drug development, utilizing advanced scientific techniques to foresee potential adverse effects of drug candidates. By leveraging in vitro, in silico, and in vivo approaches, predictive toxicology aims to provide early insights into the safety profile of drug candidates, enabling efficient decision-making in drug development. This proactive approach not only mitigates risks but also reduces the likelihood of late-stage failures, saving time and resources.
Enzo’s broad range of scientific expertise and industry-proven manufacturing capabilities provides a comprehensive set of solutions for predictive toxicology when determining genotoxicity, membrane integrity, autophagy, and oxidative stress. Our products are reliable, cost-effective, and compatible with high-throughput capabilities.
With over 45 years of experience and manufacturing excellence, Enzo’s overarching goal is to provide innovative tools that enhance the efficiency, cost-effectiveness, and overall success of drug development endeavors
Enzo's products and services can help to streamline operations, making drug development more accessible and efficient.
Cell viability testing is crucial for evaluating the impact of compounds on living cells, providing essential insights for safer and more effective drug development.
Understanding cellular dysfunction is crucial in assessing drug candidate safety. Early identification of compounds inducing dysfunction allows for timely adjustments in drug development.
The presence of aggresomes may indicate cellular stress and dysfunction, allowing for the early identification of potential toxic effects induced by drugs or other substances.
Identifying genotoxic drug compounds during early preclinical studies allows for further lead refinement and lower drug attrition.
Focused investigation helps identify and mitigate risks associated with drug-induced harm to specific organs, contributing to the development of safer and more targeted pharmacological interventions.
A comprehensive understanding of drug-induced endocrine and metabolism abnormalities is essential for predicting adverse reactions, guiding drug design, and optimizing treatment strategies.
Evaluating the health of cells through viability testing is fundamental. This essential process provides valuable insights into how compounds may affect the well-being and functionality of living cells, contributing significantly to the quest for a safer and a more effective drug development.
Utilizing Enzo’s advanced in vitro assays, potential toxicity of drug candidates can be assessed early on.
Distinguishing between apoptosis and necrosis provides valuable insights into the specific pathways through which a drug induces cell death, aiding researchers in assessing the safety and efficacy of potential drug candidates.
Multiplex Healthy, Early Apoptotic, Late Apoptotic, and Necrotic Cells
GFP-CERTIFIED® Apoptosis/Necrosis Detection Kit (ENZ-51002) detects four distinct cell states. Mitochondrial GFP-expressing HeLa cells were treated with 2μM Staurosporine for 4 hours. The apoptosis detection reagent (gold) and necrosis detection reagent (red) specifically detect cell states with clear spectral separation from mitochondria-associated GFP signal. Healthy cells (A), cells undergoing apoptosis (B), cells undergoing late-stage apoptosis (C), and necrotic cells (D).
Understanding how drug candidates may impact critical cellular processes, aids in the prediction of the compound's overall effect on the cell. Compounds that induce significant dysfunction may pose risks to overall organism health, and early identification allows for appropriate adjustments in drug development.
Enzo’s CELLESTIAL® Assays for drug safety & in vitro toxicology provide a comprehensive set of solutions for monitoring cellular processes, aiding in tailoring drug development strategies to enhance therapeutic effectiveness.
New drugs or compounds can selectively affect mitochondrial processes, such as membrane potential, reactive oxygen species (ROS) production, and calcium homeostasis, thus contributing to toxicity. Enzo’s MITO-ID® Membrane Potential Cytotoxicity Kit is a real-time mitochondrial membrane potential assay suitable for time-course studies evaluating intact and compromised mitochondria.
The presence of aggresomes, or inclusion bodies of misfolded and/or aggregated proteins, is often linked to cellular toxicity. Aggresomes may indicate cellular stress and dysfunction, allowing for the early identification of potential toxic effects induced by drugs or other substances.
Detect and quantify aggresomes in cells using PROTEOSTAT® Aggresome Detection Kit, a robust and highly sensitive assay for toxicity assessment during drug discovery and development.
Identifying genotoxic drug compounds during early preclinical studies allows for further lead refinement and lower drug attrition. Mutagenesis, carcinogenesis, and adverse genetic effects, especially those that affect the germline, are routinely tested. Common assays for mutagenicity, both in vitro and in vivo, identify mutagenic hazards and mitigate risk for regulatory approval.
Exposure of cells to oxidative and environmental stresses frequently results in the breakdown or oxidation of genomic DNA. DNA Damage ELISA kit is a colorimetric solution to evaluate the integrity of genomic DNA, or to assess the presence of oxidized DNA and is frequently used as means of verifying the onset of apoptosis or DNA damage.
Sensitive measurement of DNA Damage
Standard curve of DNA damage ELISA kit detecting as low as 1 ng/ml of 8-OHdG
Understanding the potential toxicities directed at specific organs is paramount for ensuring the safety and efficacy of a drug candidate. This focused investigation allows to identify and mitigate risks associated with drug-induced harm to particular organs, contributing to the development of safer and more targeted pharmacological interventions.
Enzo's SCREEN-WELL® compound libraries are a unique collection of focused small molecules with defined and diverse organ-associated toxicity profiles. A variety of structurally and mechanistically different compound classes is included, as well as non-toxic controls.
Tamoxifen
Tamoxifen, a compound included in Enzo’s SCREEN-WELL® Hepatotoxicity Library, is a selective estrogen receptor modulator (SERM), is commonly used in the treatment of hormone receptor-positive breast cancer. Considered generally safe and effective, it can occasionally cause hepatotoxicity. Tamoxifen and its metabolites can undergo bioactivation by cytochrome P450 enzymes, leading to the formation of reactive intermediates that can damage liver cells.
Drug-induced endocrine and metabolism disorders include electrolyte and calcium abnormalities, carbohydrate metabolism, and thyroid and gonadal disorders. A critical understanding of endocrine effects can better predict drug-induced adverse effects for a better choice of drug design and treatment rationale.
Monitoring cortisol levels can provide valuable insights into the impact of drugs on the endocrine system and metabolic homeostasis. Changes in cortisol levels may indicate stress, alterations in energy metabolism, or potential adverse effects on the adrenal glands.
Accurately Detect Cortisol with Exceptional Consistency Across Multiple Production Lots
Lot-to-lot consistency graph demonstrates the robust and reproducible nature of the Cortisol ELISA kit showing standard curves from 8 lots manufactured over 7 years.
Reduce the risk of your drug discovery pipeline with our cell-based assays, activity kits, and reagents for mechanism and pathway analysis and biomarker detection. Enzo’s broad range of scientific expertise and industry-proven manufacturing capabilities provides a comprehensive set of solutions for the toxicology market when determining cell viability, cellular dysfunction, genotoxicity, organ-specific toxicity and metabolic abnormalities
Enzo's offerings and solutions are designed to enhance operational efficiency, simplifying processes and increasing accessibility in the field of drug development.
