Collagenases & Gelatinases

Evaluate Extracellular Matrix Proteins & Metalloproteinases

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Extensive Collagenase & Gelatinase Product Range

Collagenases (Matrix Metalloproteinase-1, MMP-8, and MMP-13) and gelatinases (MMP-2 and MMP-9) break down skin collagens, contributing to aging and wrinkling. Enzo offers high sensitivity colorimetric and fluorometric activity assays for screening collagenase and gelatinase inhibitors. Each assay is built using high purity active enzymes, and includes substrate, 96-well plate, and protocol.

	Product Types
	Assay Kits	Substrates	Active Proteins	Antibodies	Inhibitors
Broad Spectrum MMP Analysis	Find	Find	Find		Small Molcules / TIMPs
MMP-1	Find	Find	Find	Find
MMP-2	Find	Find	Find	Find	Find
MMP-3	Find	Find	Find	Find	Find
MMP-7	Find	Find	Find	Find
MMP-8	Find	Find	Find
MMP-9	Find	Find	Find	Find
MMP-10	Find	Find	Find	Find
MMP-11		Find	Find
MMP-12	Find	Find	Find	Find
MMP-13	Find	Find	Find	Find
MMP-14	Find	Find	Find
MMP-19	Find	Find	Find
MMP-20	Find	Find	Find
MMP-24		Find	Find

MMP Assay Kits

Optimized Detection of MMP Inhibitors

Matrix Metalloproteinase (MMP) Inhibitor Profiling Kit Fluorometric RED

Inhibition of MMPs has been a target for inflammation, wound healing, skin aging, skin tone discoloration, rosacea, acne, and hair growth modulation. Our OmniMMP™ RED substrate offers key advantages over other substrates used for screening MMP inhibitors.

Emission spectrum avoids the interference at lower wavelengths often exhibited by certain screening compounds
Ultra-strong fluorescence allows for substrate concentrations much lower than the Km, which is desirable for inhibitor screening assays
Superior solubility characteristics compared with other MMP substrates
Convenient real-time kinetics of cleavage is easily determined
Includes active recombinant enzyme, substrate, and assay buffer

Inhibitor Dose-response Curve

NNGH was pre-incubated with MMP enzyme before reactions were started by the addition of substrate.

Screen For Inhibitors of Specific MMPs

Matrix Metalloproteinase-1 (MMP-1) Fluorometric Drug Discovery Kit, RED

Flexible platforms available for colorimetric and fluorometric detection
Sensitive detection for specific broad range of MMPs
Includes high quality substrate, enzyme and prototypic control inhibitor

Determination of k_cat/K_m for MMP-1 using Omni MMP™ RED and Km=0.48 μM, k_cat/K_m=6.7x10⁶ M^-1 sec

MMP Inhibitors

Actinonin

Known inhibitor of select MMPs (-1, -2, -3, -7, -8, -9, -10, -12, -13)
High purity, ≥98%
Widely cited for various applications involving its inhibitory and antibiotic properties