Comprehensive Tools for Epigenetic Modification

Epigenetics can be defined as the structural adaptation of chromosomal regions so as to register, signal or perpetuate altered activity states. A variety of chromatin-altering post-translational modifications (PTMs) to histone proteins including acetylation, methylation, and ubiquitinylation, as well as direct modifications to DNA, are known to turn gene transcription off or on. To date, acetylation/deacetylation of histone lysine residues, and methylation of DNA have proven to be of greatest clinical significance amongst epigenetic PTMs.

The approval of anti-cancer drugs such as Vorinostat and Decitabine, targeting Histone Deacetylases (HDACs) and DNA Methyltransferases (DNMTs), respectively, as well as pharma development pipelines targeting PTM regulating enzymes, are evidence that reprograming gene expression on an epigenetic level holds great clinical promise.

NEW! HDAC/SIRT Chemiluminescent Drug Discovery Kit

Based on our patent-pending Chemilum de Lys™ substrate and developer combination, the HDAC/SIRT Chemiluminescent Drug Discovery Kit provides a chemiluminescent alternative to radiolabeled and HPLC methods for HDAC activity. The simple 3-step assay procedure is designed to measure HDAC and Sirtuin activity in cellular or nuclear extracts, immunoprecipitates, or using purified enzymes.

• Eliminate screening artifacts encountered with luciferase-coupled assays or autofluorescent compounds
• Glow-type luminescence provides a stable signal (10-min half-life) readily detected with a luminometer
• High signal-to-background ratios and excellent Z´-factor scores are suitable for HTS screening
• High-throughput format validated with nuclear extract and 10 different recombinant HDACs and Sirtuins

Could A Little Ginger Help Fight Cancer?
2013 April 5 | Tags: Autophagy, Cancer, Epigenetics, Proteasome

The Phytochemical Sulforaphane Appears to Prevent Cancer through Two Distinct Epigenetic Mechanisms.
2012 September 21 | Tags: Cancer, Epigentics

Chemical Genetic Approach Confirms Metabolic Benefits of Sirt1.
2012 August 24 | Tags: Cancer, Epigentics, Metabolism

Resveratrol's metabolic effects include inhibition of PDE 4 and activation of sirtuin 1.
2012 April 13 | Tags: Cancer, Epigenetics, Inflammation, Metabolism, Neuroscience