Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

BIOLIKE® Conformational ELISA Kits

Building the Bridge to Development



BIOLIKE® Conformational ELISA Kits

The BIOLIKE® Conformational ELISA kits allow for 3-D conformational comparison, higher-order structure (HOS) characterization, between biosimilars and marketed mAbs. This new technology provides a sensitive, systematic, and robust measurement of biosimilar conformation comparability at the molecular level.


Applications

  • Monoclonal Antibody Development
  • Antibody-Drug Conjugates (ADCs)
  • Cell Line Selection
  • Process Development
  • Formulation Development
  • Comparability Studies
  • Product Characterization
 

How it works

The assay uses more than 30 polyclonal antibodies to cover an entire mAb, thereby measuring its surface –epitope distribution systematically and sensitively, providing a unique measurement of biosimilar mAb HOS Comparability.

The panel of antibodies is raised against peptides derived from the full length protein sequence of a biologic. Taken individually, each antibody is strongly antigenic to the peptide sequence it was produced from. When peptides are in a full length correctly folded protein, the antigenicity is masked by 3D structure and only a limited number of antibodies respond.

A histogram of binding to the antibody panel can be likened to a “fingerprint” for the correctly folded biologic. If a Biosimilar is correctly folded and glycosylated, the fingerprint will match that of the biologic. If the Biosimilar is not correctly folded, previously masked peptide sequences will be exposed and will be recognized by the Ab made to that exposed sequence.


Key Features

  • Unique measurement of biosimilar mAb HOS Comparability.
  • Detects small amounts of conformational impurity, ≥ 0.1%.
  • Correlates well with bioassays and glycosylation analysis.
 

Featured Product

BIOLIKE® Conserved mAb sequence conformational ELISA kit

Systematic and sensitive immunoassay to monitor the structural conformation of a novel mAb during the development process.

  • Uses a panel of more than 30 antibodies raised against commercially aa sequences of available mAb biologic drugs
  • Enables the monitoring of conformation changes throughout the mAb development process, post purification or other manipulations.

A typical ELISA comparing 2 novel mAbs in the variable region
 

All our BIOLIKE® Conformational ELISA Kits

BIOLIKE® Bevacizumab conformational ELISA kit 

Systematic and sensitive immunoassay to compare the structural conformation between Bevacizumab and a biosimilar mAb.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT118-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

BIOLIKE® Alemtuzumab conformational ELISA kit 

Systematic and sensitive immunoassay to compare the structural conformation between Alemtuzumab and a biosimilar mAb.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT119-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

BIOLIKE® Cetuximab conformational ELISA kit 

Systematic and sensitive immunoassay to compare the structural conformation between Cetuximab and a biosimilar mAb.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT120-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

BIOLIKE® Trastuzumab conformational ELISA kit 

Systematic and sensitive immunoassay to compare the structural conformation between Trastuzumab and a biosimilar mAb.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT121-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

BIOLIKE® Adalimumab conformational ELISA kit 

Systematic and sensitive immunoassay to compare the structural conformation between Adalimumab and a biosimilar mAb.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT122-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

BIOLIKE® Conserved mAb sequence conformational ELISA kit 

Systematic and sensitive immunoassay to monitor the structural conformation of a novel mAb during the development process.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT123-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

BIOLIKE® Infliximab conformational ELISA 

Systematic and sensitive immunoassay to compare the structural conformation between Infliximab and a biosimilar mAb.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT124-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

BIOLIKE® Rituximab conformational ELISA kit 

Systematic and sensitive immunoassay to compare the structural conformation between Rituximab and a biosimilar mAb.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT125-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

BIOLIKE® Palivizumab conformational ELISA kit 

Systematic and sensitive immunoassay to compare the structural conformation between Palivizumab and a biosimilar mAb.
ELISA, Colorimetric detection | Print as PDF
 
ENZ-KIT126-0003 3x96 wells 964.00 USD
Do you need bulk/larger quantities?
 

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,