C2 Ceramide

Cell-permeable ceramide analog



BML-SL100-0005	5 mg	52.00 USD

BML-SL100-0025	25 mg	207.00 USD

Replaces Prod. #: ALX-306-024

N-Acetylsphingosine is a biologically active, cell-permeable ceramide analog. It inhibits cell proliferation and induces monocytic differentiation of HL-60 cells and induces apoptosis. It stimulates protein phosphatase 2A4, activates MAP kinase and SAP kinase and induces PKCδ and ε translocation. Physiological levels of C2 ceramide, produced by the action of a PAF:sphingosine CoA-independent transacetylase, have been detected in HL-60 cells.

Product Specification

Alternative Name:	N-Acetyl-D-erythro-sphingosine

Formula:	C₂₀H₃₉NO₃

MW:	341.5

Purity:	≥98% (TLC)

Appearance:	White solid.

CAS:	3102-57-6

Solubility:	Soluble in 100% ethanol (>25mg/ml) or DMSO (>50mg/ml).

Long Term Storage:	-20°C

Use/Stability:	Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C.

Background / Technical Information:	Please click here for the comprehensive product datasheet.C2 ceramide may precipitate from aqueous media, particularly those lacking serum or serum albumin, at concentrations ~20µM or higher. To avoid this problem, a 1:1 complex of C2 Ceramide with BSA may be prepared as follows: Dissolve C2 ceramide to 200mM (68.3mg/ml) in anhydrous DMSO. Dissolve fatty acid-free BSA in water to 66mg/ml (1mM). Dilute the ceramide/DMSO stock 200-fold into the BSA solution, with constant stirring. A precipitate will form at first, but will dissolve after ~30 minutes of stirring at room temperature. Alternatively, a solution of C2 Ceramide/BSA may be prepared directly, without making a DMSO stock: 1) prepare a 20% w/v stock of BSA in water (3mM); 2) use the BSA stock to dissolve the solid C2 Ceramide at 1.02mg/ml (3mM). Complete dissolution of the ceramide may require more than 1 hour of stirring at room temperature.

Please mouse over

Product Literature References

Ceramide-mediated biology. Determination of structural and stereospecific requirements through the use of N-acyl-phenylaminoalcohol analogs: A. Bielawska, et al.; J. Biol. Chem. 267, 18493 (1992), Abstract;