C2 Ceramide
[N-Acetyl-D-erythro-sphingosine]
| BML-SL100-0005 | 5 mg | 50.00 USD |  |
|
| BML-SL100-0025 | 25 mg | 201.00 USD | |
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Replaces Prod. #: ALX-306-024
N-Acetylsphingosine is a biologically active, cell-permeable ceramide analog. It inhibits cell proliferation and induces monocytic differentiation of HL-60 cells and induces apoptosis. It stimulates protein phosphatase 2A4, activates MAP kinase and SAP kinase and induces PKCδ and ε translocation. Physiological levels of C2 ceramide, produced by the action of a PAF:sphingosine CoA-independent transacetylase, have been detected in HL-60 cells.
Product Specification
| Formula: | C20H39NO3 |
| MW: | 341.5 |
| Purity: | ≥98% (TLC) |
| Appearance: | White solid. |
| CAS: | 3102-57-6 |
| Solubility: | Soluble in 100% ethanol (>25mg/ml) or DMSO (>50mg/ml). |
| Shipping: | AMBIENT |
| Long Term Storage: | -20°C |
| Use/Stability: | Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C. |
| Background / Technical Information: | Please click here for the comprehensive product datasheet.C2 ceramide may precipitate from aqueous media, particularly those lacking serum or serum albumin, at concentrations ~20µM or higher. To avoid this problem, a 1:1 complex of C2 Ceramide with BSA may be prepared as follows: Dissolve C2 ceramide to 200mM (68.3mg/ml) in anhydrous DMSO. Dissolve fatty acid-free BSA in water to 66mg/ml (1mM). Dilute the ceramide/DMSO stock 200-fold into the BSA solution, with constant stirring. A precipitate will form at first, but will dissolve after ~30 minutes of stirring at room temperature. Alternatively, a solution of C2 Ceramide/BSA may be prepared directly, without making a DMSO stock: 1) prepare a 20% w/v stock of BSA in water (3mM); 2) use the BSA stock to dissolve the solid C2 Ceramide at 1.02mg/ml (3mM). Complete dissolution of the ceramide may require more than 1 hour of stirring at room temperature. |
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Product Literature References
Ceramide-mediated biology. Determination of structural and stereospecific requirements through the use of N-acyl-phenylaminoalcohol analogs: A. Bielawska, et al.; J. Biol. Chem. 267, 18493 (1992), Abstract;
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