Maximizing yield in biopharmaceutical production is crucial for optimizing productivity and cost-effectiveness. It involves strategic process optimization, such as enhancing cell culture conditions, optimizing fermentation parameters, and improving downstream purification techniques.

MEGACD40L® Protein (soluble)(human) (recombinant)

High-activity, high-purity CD40L construct for co-stimulatory activation of an immune response without enhancers.

• Superior purity eliminates experimental artifacts
• 100-fold lower endotoxin levels than comparable products
• Reliable results through consistent lot-to-lot performance

Achieve rapid expansion of primary B cells with MEGACD40L® recombinant protein. Using MEGACD40L® in association with other stimulants can improve expansion of primary B cells by 36 fold, based on the work by Hung KL, et al

Small Molecules and Peptides

Incorporating small molecules into bioprocessing workflows can enhance productivity at various stages of the production cycle. These molecules act as potent enhancers, catalysts, or modulators, driving improvements in yield, quality, and overall process performance.

Our chemistry and compound range includes over 3,000 stand-alone small molecules with known activity, including natural products, enzyme inhibitors, receptor ligands, drugs, lipids, and fatty acids. From metabolic engineering to downstream processing, these compounds offer unprecedented opportunities to enhance productivity, streamline workflows, and achieve greater efficiency in producing valuable biologics.

By monitoring aggregation, product stability can be assessed, allowing for the optimization of formulation and manufacturing processes to meet stringent regulatory requirements. This proactive approach ensures the final biopharmaceutical product maintains its integrity, efficacy, and safety.

PROTEOSTAT® Protein Aggregation Assay

Based on an innovative molecular rotor dye, the PROTEOSTAT® Protein Aggregation Assay is a simple, sensitive, and homogeneous fluorescent microplate assay allowing the detection in solution of a broad range of protein aggregates, from visible to subvisible particles.

• Delivers superior results when compared to conventional protein detection dyes
• High-throughput method for monitoring protein stability when used with flow cytometry or microplate reader
• Provides a convenient, complementary orthogonal method for cross-validation of instrumentation-intensive techniques
• Useful assay to troubleshoot protein damage, from protein development through manufacturing stages

Related Product
PROTEOSTAT® Protein Aggregation Standards

Reference articles

Optimizing Biologics Formulations. Here and There on Upstream Processing.

PROTEOSTAT® Thermal Shift Stability Assay

Based on an innovative molecular rotor dye, the PROTEOSTAT® Thermal Shift Stability Assay is a rapid, simple, and sensitive screening method allowing the end user to investigate proteins’ aggregation propensity by determining the aggregation temperature.

• Perform accelerated screening for protein stability as a function of pH, ionic strength, and concentration
• Screen for ligand binding to proteins of unknown function
• Identify protein-protein interaction inhibitors

Reference article

Here and There on Upstream Processing.

Contamination in biopharmaceuticals can occur at different stages of production and includes sources such as microorganisms, adventitious agents, and chemical impurities. Effective monitoring and control play a critical role in ensuring the safety and integrity of biopharmaceutical products while meeting rigorous regulatory standards.

Protein A ELISA Kit

Protein A ELISA kit is a sensitive and reproducible sandwich immunoassay to quantify Protein A residuals in monoclonal antibody preparations.

• Highly sensitive measurement, detecting as little as 9.01 pg/ml (<1 ppm) of Protein A residuals in purified humanized mAb preparations
• Universally used as it recognizes 4 commonly used constructs of Protein A
• High-throughput format with results in under 3 hours for up to 37 samples in duplicate

Host Cell Protein ELISA Kits

The Host Cell Protein ELISA kits and reagents are ideal for impurity analysis. Quantitatively measure host cell protein contamination in E.coli, CHO, and HEK293T derived products.

• Sensitive measurement of host cell protein, detecting as little as 10 ng/ml
• High protein coverage greater than 70% thereby, reducing the risk of missing potential contaminants
• High-throughput format with results in as little as 3 hours

CHO Host Cell Protein ELISA kit
E.coli Host Cell Protein ELISA kit
HEK293T Host Cell Protein ELISA kit

Related Product
CHO HCP Standards
E.coli HCP Standards
HEK293T Host Cell Protein Standards