Dnmt1, mAb (60B1220.1)

[DNA methyltransferase-1]



ALX-804-369-C100	100 µg	293.00 USD

Product Specification

Concentration:	0.5mg/ml

Purity Detail:	Protein G-affinity purified.

Formulation:	Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.

Clone:	60B1220.1

Isotype:	Mouse IgG1

Immunogen:	Synthetic peptide corresponding to aa 637-650 of human Dnmt1 (DNA methyltransferase-1).

Specificity:	Recognizes human, mouse and zebrafish Dnmt1.

Application:	Immunocytochemistry (see [14]) Immunohistochemistry (1-2µg/ml) (paraffin sections (see [17])) Immunoprecipitation (see [3,7]), Chromatin Immunoprecipitation (see [11])) Western Blot (2-4µg/ml)

Shipping:	SHIPPED ON BLUE ICE

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Use/Stability:	Stable for 6 months when stored at +4°C.

Handling:	Avoid freeze/thaw cycles.

Background / Technical Information:	Replacement for ADI-KAM-TF040

Figure: Western blot analysis for Dnmt1 using ALX-804-369 at 2µg/ml dilution against 10µg of cell lysates of mouse ES cell.

Figure 2: Immunohistochemistry of Dnmt1 using ALX-804-369 at 2µg/ml on formalin-fixed paraffin-embedded medullar kidney tissue sections.

Please mouse over

Product Literature References

Lsh, a member of the SNF2 family, is required for genome-wide methylation: K. Dennis, et al.; Genes Dev. 15, 2940 (2001), Abstract; Full Text

Age-dependent DNA methylation changes in the ITGAL (CD11a) promoter: Z. Zhang, et al.; Mech. Ageing Dev. 123, 1257 (2002), Abstract;

Farnesyltransferase inhibitor (L-744,832) restores TGF-beta type II receptor expression and enhances radiation sensitivity in K-ras mutant pancreatic cancer cell line MIA PaCa-2: R.A. Alcock, et al.; Oncogene 21, 7883 (2002), Abstract;

The Epstein-Barr virus oncogene product, latent membrane protein 1, induces the downregulation of E-cadherin gene expression via activation of DNA methyltransferases: C.N. Tsai, et al.; PNAS 99, 10084 (2002), Abstract; Full Text

The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription: R. Santoro, et al.; Nat. Genet. 32, 393 (2002), Abstract;

Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns: J.M. Craig, et al.; Hum. Mol. Genet. 12, 3109 (2003), Abstract; Full Text

Decrease of DNA methyltransferase 1 expression relative to cell proliferation in transitional cell carcinoma: F. Kimura, et al.; Int. J. Cancer 104, 568 (2003), Abstract;

Double RNA interference of DNMT3b and DNMT1 enhances DNA demethylation and gene reactivation: Y.W. Leu, et al.; Cancer Res. 63, 6110 (2003), Abstract;

Components of a pathway maintaining histone modification and heterochromatin protein 1 binding at the pericentric heterochromatin in Mammalian cells: H. Xin, et al.; J. Biol. Chem. 279, 9539 (2004), Abstract; Full Text

Induction of DNA methylation and gene silencing by short interfering RNAs in human cells: H. Kawasaki and K. Taira; Nature 431, 211 (2004), Abstract;

Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res. 64, 8184 (2004), Abstract;

RNA interference-mediated knockdown of DNA methyltransferase 1 leads to promoter demethylation and gene re-expression in human lung and breast cancer cells: M. Suzuki, et al.; Cancer Res. 64, 3137 (2004), Abstract; Full Text

5-Aza-deoxycytidine induces selective degradation of DNA methyltransferase 1 by a proteasomal pathway that requires the KEN box, bromo-adjacent homology domain, and nuclear localization signal: K. Ghoshal, et al.; Mol. Cell. Biol. 25, 4727 (2005), Abstract;

An association of DNMT3b protein expression with P16INK4a promoter hypermethylation in non-smoking female lung cancer with human papillomavirus infection: T.S. Lin, et al.; Cancer Lett. 226, 77 (2005), Abstract;

Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development: I.Y. Park, et al.; J. Cell Biochem. 94, 585 (2005), Abstract;

Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma: B.I. Srivastava and M.D. Srivastava; Leuk. Res. 29, 771 (2005), Abstract;

Immunology of cutaneous vasculitis associated with both etanercept and infliximab: M.D. Srivastava, et al.; Scand. J. Immunol. 61, 329 (2005), Abstract;