DAF-FM DA (cell permeable)

[Diaminofluorescein-FM . diacetate]



ALX-620-071-M001	1 mg	570.00 USD

Cell permeable analog of DAF-FM (Prod. No. ALX-620-070), that is hydrolyzed to DAF-FM by intracellular esterases (Ex: 500nm; Em: 515nm). Stable in a pH range of 5.5-12. High yield (quantum efficiency). Detection limit 3nM. Photostable. Not for sale in Japan.

Product Specification

Concentration:	~5mM

Formula:	C₂₅H₁₈F₂N₂O₇

MW:	496.4

Purity:	≥98% (HPLC)

Formulation:	Liquid. In 0.4ml DMSO.

Shipping:	SHIPPED ON BLUE ICE

Long Term Storage:	-20°C

Use/Stability:	Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.

Handling:	After opening, prepare aliquots and store at -20°C. Keep under inert gas. Protect from light.

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Product Literature References

Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry: Y. Itoh, et al.; Anal. Biochem. 287, 203 (2000), Abstract;

Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000),

Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem. 73, 1967 (2001), Abstract;

Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia: H. Kojima, et al.; J. Neurochem. 76, 1404 (2001), Abstract;

Imaging of nitric oxide in a living vertebrate using a diamino-fluorescein probe: S. Lepiller et al.; Free Radic. Biol. Med. 43, 619 (2007), Abstract;