Th-POK (human), pAb
[T-helper-inducing POZ/Krueppel-like factor, Zinc finger protein 67 homolog, ZFP67, Zinc finger and BTB domain-containing protein 7B, ZBTB7B]
| ALX-210-471-C100 | 100 µg | 289.00 USD |  |
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Product Specification
| Concentration: | 1mg/ml. |
| Purity Detail: | Purified IgG. |
| Formulation: | Liquid. In PBS, pH 7.4. Contains no preservatives. |
| Immunogen: | Recombinant human Th-POK. |
| Source/Host: | From rabbit |
| Specificity: | Recognizes human Th-POK. |
| Application: | ELISA (direct and indirect: 1:2’000-1:5’000) Immunohistochemistry (paraffin sections (5µg/ml)) Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
| Shipping: | SHIPPED ON BLUE ICE |
| Short Term Storage: | +4°C |
| Long Term Storage: | -20°C |
| Use/Stability: | Stable for 6 months after receipt when stored at -20°C. |
| Handling: | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
Figure 1: Western blot analysis of human Th-POK using PAb to Th-POK (human) (Prod. No. ALX-210-471) at 1:2’000 dilution. 1. Recombinant human Th-POK. 2. Transfected human Th-POK cell lysate (HEK 293). 3. Mock Transfected HEK293 cell lysate. 4. Recombinant human His-tagged omentin 1 (Prod. No. ALX-201-371) (negative control).
Figure 2: Immunohistochemistry of human Th-Pok expression using PAb to Th-POK (human). Method: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human uterus tissue section (normal cervix, Pantomics, Inc. USA) using PAb to Th-POK (human) (Prod. No. ALX-210-471) at 5µg/ml shows nuclear and cytoplasmic staining (40x, brown color). Small picture (right): Immunohistochemical staining using other rabbit PAb in human uterus (negative control).
Figure 3: Immunohistochemistry of human Th-Pok expression using PAb to Th-POK (human).Method: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human small intestine tissue section (Pantomics, Inc. USA) using PAb to Th-POK (human) Prod. No. ALX-210-471) at 5µg/ml shows nuclear and cytoplasmic staining (40X, brown color). Small picture (right): Immunohistochemical staining using other rabbit PAb in human small intestine (negative control).
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