This kit is an enzyme-linked immunosorbent assay (ELISA) used for the quantitative determination of intracellular sirtuin 1 in human cell lysates.
Product Specification
| Sensitivity: | 30pg/ml |
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| Specificity: | Does not cross-react with human sirtuin 2, 5, and 6. |
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| Application: | For the quantitative determination of intracellular sirtuin 1 in human cell lysates. |
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| Shipping: | SHIPPED ON BLUE ICE |
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| Long Term Storage: | +4°C |
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| Use/Stability: | Diluted wash solution may be stored at room temperature for up to one month. |
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| Miscellaneous/General: | Sirtuin1 is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates Lys-382 of p53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD+/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates Lys-16 of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at Lys-9 (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. |
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| Background / Technical Information: | Swiss-Prot link Q96EB6: SIRT1 (human) |
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