Sirtuin 1 (human) (IntraCellular) ELISA Kit
[Sirt01, SIR02 like Protein 01]
| AG-45A-0029EK-KI01 | 96 wells | 670.00 USD |  |
|
| AG-45A-0029TP-KI01 | 2x96 wells | Twin Plex | 980.00 USD | |
This kit is an enzyme-linked immunosorbent assay (ELISA) used for the quantitative determination of intracellular sirtuin 1 in human cell lysates.
Product Specification
| Sensitivity: | 30pg/ml |
| Specificity: | Does not cross-react with human sirtuin 2, 5, and 6. |
| Application: | For the quantitative determination of intracellular sirtuin 1 in human cell lysates. |
| Shipping: | SHIPPED ON BLUE ICE |
| Long Term Storage: | +4°C |
| Use/Stability: | Diluted wash solution may be stored at room temperature for up to one month. |
| Miscellaneous/General: | Sirtuin1 is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates Lys-382 of p53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD+/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates Lys-16 of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD+/NADP+ ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at Lys-9 (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein. |
| Background / Technical Information: | Swiss-Prot link Q96EB6: SIRT1 (human) |
Product Toolbox
PRODUCT RESOURCES
Datasheet
Manual
RELATED PRODUCTS
By catalog section:
PRODUCT SUPPORT
Technical ServiceBulk Quantity Quote
Customer Service
