Sirtuin 1 (human) (IntraCellular) ELISA Kit



AG-45A-0029EK-KI01	96 wells		660.00 USD

AG-45A-0029TP-KI01	2x96 wells	Twin Plex	950.00 USD

This kit is an enzyme-linked immunosorbent assay (ELISA) used for the quantitative determination of intracellular sirtuin 1 in human cell lysates.

Product Specification

Alternative Name:	Sirt01, SIR02 like Protein 01

Sensitivity:	30pg/ml (range 0.032 to 2ng/ml)

Specificity:	Does not cross-react with human sirtuin 2, 5, and 6.

Application:	For the quantitative determination of intracellular sirtuin 1 in human cell lysates.

Long Term Storage:	+4°C

Use/Stability:	Diluted wash solution may be stored at room temperature for up to one month.

Miscellaneous/General:	Sirtuin1 is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Deacetylates Lys-382 of p53 and impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylates TAF1B and thereby represses rDNA transcription by the RNA polymerase I. Involved in HES1- and HEY2-mediated transcriptional repression. Inhibits skeletal muscle differentiation by deacetylating PCAF and MYOD1. May serve as a sensor of the cytosolic ratio of NAD/NADH, which is essential in skeletal muscle cell differentiation. Deacetylates Lys-16 of histone H4 (in vitro). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD /NADP ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at Lys-9 (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In case of HIV-1 infection, interacts with and deacetylates the viral Tat protein.

Background / Technical Information:	UniProt ID Q96EB6: SIRT1 (human)

Manufacturer:	Manufactured by AdipoGen, Inc.