Alternative size available: ADI-900-164 (1x96 wells)
Versatile and convenient kit to assay both intracellular and extracellular cGMP
High sensitivity with an optional acetylation protocol which increases sensitivity 10-fold (0.059 pmol/ml)
Reliable and consistent lot-to-lot performance
Higher throughput format with results in just 3 hours for up to 39 samples in duplicate
Simple, efficient and well-established sample handling protocols
Save with bulk kit package!
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP diluted in buffer or intracellular cGMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm. The ready-to-use liquid color-coded reagents provided reduce error.
Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.
Effects of a Soluble Guanylate Cyclase Stimulator Riociguat on Contractility of Isolated Pulmonary Artery and Hemodynamics of U46619-Induced Pulmonary Hypertension in Dogs: S. Kameshima, et al.; Vet. Sci. 10, 159 (2023), Abstract;
A Series of Substituted Bis-Aminotriazines Are Activators of the Natriuretic Peptide Receptor C: R.J. Smith, et al.; J. Med. Chem. 65, 5495 (2022), Abstract;
Broadening the Spectrum of Loss-of-Function Variants in NPR-C-Related Extreme Tall Stature: P. Lauffer, et al.; J. Endocr. Soc. 6, bvac019 (2022), Abstract;
Evaluation of Multivalent Enterotoxigenic Escherichia coli Vaccine Candidate MecVax Antigen Dose-Dependent Effect in a Murine Model: H. Seo, et al.; Appl. Environ. Microbiol. 88, e0095922 (2022), Abstract;
Novel NPR2 Gene Mutations Affect Chondrocytes Function via ER Stress in Short Stature: Q. Li, et al.; Cells 11, 1265 (2022), Abstract;
Purification, characterization, and preliminary serial crystallography diffraction advances structure determination of full-length human particulate guanylyl cyclase A receptor: S. Zhang, et al.; Sci. Rep. 12, 11824 (2022), Abstract;
Selective PDE1 inhibition ameliorates vascular function, reduces inflammatory response, and lowers blood pressure in ageing animals: K. Golshiri, et al.; J. Pharmacol. Exp. Ther. 378, 173 (2022), Abstract;
Vaccine Candidate Double Mutant Variants of Enterotoxigenic Escherichia coli Heat-Stable Toxin: E.D. Zegeye, et al.; Vaccines 10, 241 (2022), Abstract;
Discovery of small molecule guanylyl cyclase A receptor positive allosteric modulators: S.J. Sangarlingham, et al.; PNAS 118, e210938611 (2021), Abstract;
Nitric oxide attenuated transforming growth factor-β induced myofibroblast differentiation of human keratocytes: J.H. Park, et al.; Sci. Rep. 11, 41598 (2021), Abstract; Full Text
A novel NPR2 mutation (p.Arg388Gln) in a patient with acromesomelic dysplasia, type Maroteaux: N. Amano, et al.; Clin. Pediatr. Endocrinol. 29, 99 (2020), Abstract; Full Text
Arginase inhibitor, Nω-hydroxy-L-norarginine, spontaneously releases biologically active NO-like molecule: Limitations for research applications: T.Y. Momma, et al.; Free Radic. Biol. Med. 152, 74 (2020), Abstract;
Chronic inhibition of nitric oxide synthase modulates calcium handling in rat heart: I. Özakca & A.T. Özcelikay; Can. J. Physiol. Pharmacol. 97, 313 (2019), Abstract;
Argininosuccinic aciduria fosters neuronal nitrosative stress reversed by Asl gene transfer: J. Baruteau, et al.; Nat. Commun. 9, 3505 (2018), Abstract; Full Text
Melatonin protects diabetic heart against ischemia-reperfusion injury, role of membrane receptor-dependent cGMP-PKG activation: L.M. Yu, et al.; Biochim. Biophys. Acta 1864, 563 (2017), Abstract;
An analogue of atrial natriuretic peptide (C-ANP4-23) modulates glucose metabolism in human differentiated adipocytes: F.J. Ruiz-Ojeda, et al.; Mol. Cell. Endocrinol. 431, 101 (2016), Abstract;
cGMP/PKG Signaling Suppresses Inositol 1,4,5-Trisphosphate Receptor Phosphorylation and Promotes Endoplasmic Reticulum Stress in Photoreceptors of CNG Channel-Deficient Mice: H. Ma, et al.; J. Biol. Chem. 290, 20880 (2015), Application(s): ELISA using mouse retinal lysate, Abstract; Full Text
Heterozygous NPR2 mutations cause disproportionate short stature, similar to Léri-Weill dyschondrosteosis: A. Hisado-Oliva, et al.; J. Clin. Endocrinol. Metab. 100, E1133 (2015), Application(s): ELISA using primate supernatant, Abstract; Full Text
Subcellular Trafficking of Guanylyl Cyclase/Natriuretic Peptide Receptor-A with Concurrent Generation of Intracellular cGMP: I. Mani, et al.; Biosci. Rep. 35, e00260 (2015), Application(s): cGMP ELISA using human cells, Abstract; Full Text
cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland: H.R. De Jonge, et al.; Am. J. Physiol. Cell Physiol. 306, C343 (2014), Application(s): ELISA using dogfish shark supernatant, Abstract; Full Text
cGMP accumulation causes photoreceptor degeneration in CNG channel deficiency: evidence of cGMP cytotoxicity independently of enhanced CNG channel function: J. Xu, et al.; J. Neurosci. 33, 14939 (2013), Application(s): ELISA using mouse retinal lysate, Abstract; Full Text
The G Protein Coupled Receptor 3 Is Involved in cAMP and cGMP Signaling and Maintenance of Meiotic Arrest in Porcine Oocytes: C.R. Yang, et al.; PLoS One 7, e38807 (2012), Application(s): Porcine oocyte lysates, Abstract; Full Text
