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Easy-to-use color-coded reagents and pre-coated plates reduce errors and save time in the lab
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High throughput format, obtain results from up to 39 samples in duplicate in just 5 hours
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Highly sensitive assay, measure as little as 146 pg/ml
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Reliable and reproducible results day-after-day and lot-after-lot
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Save with bulk kit package!
The 12(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded reagents that reduce error.
Product Details
| Alternative Name: | 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid |
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| Sensitivity: | 146 pg/ml (range 195 - 50,000 pg/ml) |
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| Assay Time: | 5 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of 12(S)-HETE in culture supernatants and plasma from any species. Cited sample types include serum, renal interstitial fluid and tissue. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Use/Stability: | Store all components at +4°C, except standard and conjugate at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Development of heart failure with preserved ejection fraction in type 2 diabetic mice is ameliorated by preserving vascular function: M. Otto, et al.; Life Sci.
284, 119925 (2021),
Abstract;
Leukotriene B4 receptor-2 contributes to KRAS-driven lung tumor formation by promoting interleukin-6-mediated inflammation: J.H. Jang, et al.; Exp. Mol. Med.
53, 1559 (2021),
Abstract;
12(S)-HETE Mediates Diabetes Induced Endothelial Dysfunction by Activating Intracellular Endothelial Cell TRPV1: M. Otto, et al.; J. Clin. Invest.
130, 4999 (2020),
Abstract;
Full Text
Arachidonate 12-Lipoxygenase and 12-Hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer: E.H. Sarsour, et al.; J. Biol. Chem.
295, 6946 (2020),
Abstract;
Full Text
A Mediterranean Diet Rich in Extra-Virgin Olive Oil Is Associated with a Reduced Prevalence of Nonalcoholic Fatty Liver Disease in Older Individuals at High Cardiovascular Risk: X. Pintó, et al.; J. Nutr.
149, 1920 (2019),
Application(s): Human Urine,
Abstract;
Ablation of TRPV1 Elevates Nocturnal Blood Pressure in Western Diet-fed Mice: B. Zhong, et al.; Curr. Hypertens. Rev.
15, 144 (2019),
Application(s): Quantification of 12(S)-HETE in Urine,
Abstract;
Full Text
Mediatory roles of leukotriene B4 receptors in LPS-induced endotoxic shock: S.Y. Kwon, et al.; Sci. Rep.
9, 5936 (2019),
Application(s): Mouse peritoneal lavage fluid and serum,
Abstract;
Full Text
5‐/12‐Lipoxygenase‐linked cascade contributes to the IL‐33‐induced synthesis of IL‐13 in mast cells, thus promoting asthma development: M. Ro, et al.; Allergy
73, 350 (2018),
Application(s): Cell Culture Supernatant,
Abstract;
Intravasation of SW620 colon cancer cell spheroids through the blood endothelial barrier is inhibited by clinical drugs and flavonoids in vitro: S. Holzner, et al.; Food Chem. Toxicol.
111, 114 (2018),
Application(s): ELISA using culture supernatants,
Abstract;
Mice lacking L-12/15-lipoxygenase show increased mortality during kindling despite demonstrating resistance to epileptogenesis: M.A. Kanzler, et al.; Epilepsia Open
3, 255 (2018),
Application(s): Cell Culture Supernatant,
Abstract;
Full Text
Changes in platelet function with inflammation in patients undergoing vascular surgery: B. Olechowski, et al.; Platelets
30, 190 (2017),
Application(s): Human Blood Serum,
Abstract;
12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci.
17, E684 (2016),
Application(s): Measurement of HETE levels in rats,
Abstract;
Full Text
Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching: C.H Nguyen, et al.; Br. J. Cancer
115, 364 (2016),
Application(s): 12(S)-HETE assay, rabbit,
Abstract;
Full Text
12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res.
56, 502 (2015),
Application(s): ELISA,
Abstract;
Full Text
Lobatin b inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation: I. Kiss, et al.; Cancer Lett.
356, 994 (2015),
Application(s): ELISA using MCF7 cell culture supernatants,
Abstract;
MyD88-BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage: A.J. Lee, et al.; Exp. Mol. Med.
47, e156 (2015),
Application(s): ELISA using macrophage incubations,
Abstract;
Full Text
Selective inhibition of 12-lipoxygenase protects islets and beta cells from inflammatory cytokine-mediated beta cell dysfunction: D.A. Taylor-Fishwick, et al.; Diabetologia
58, 549 (2015),
Abstract;
Transient postnatal fluoxetine decreases brain concentrations of 20-HETE and 15-epi-LXA4, arachidonic acid metabolites in adult mice: Z.X. Yuan, et al.; Prostaglandins Leukot. Essent. Fatty Acids
101, 9 (2015),
Application(s): ELISA using mouse brain samples,
Abstract;
12-Lipoxygenase promotes obesity-induced oxidative stress in pancreatic islets: S.A. Tersey, et al.; Mol. Cell. Biol.
34, 3735 (2014),
Application(s): ELISA using culture supernatants,
Abstract;
The oxido-reductase enzyme glutathione peroxidase 4 (GPX4) governs Salmonella Typhimurium-induced neutrophil transepithelial migration: T.A. Agbor, et al.; Cell. Microbiol.
16, 1339 (2014),
Application(s): ELISA using culture supernatants,
Abstract;
Full Text
In vitro inhibition of breast cancer spheroid-induced lymphendothelial defects resembling intravasation into the lymphatic vasculature by acetohexamide, isoxsuprine, nifedipin and proadifen: N. Kretschy, et al.; Br. J. Cancer
108, 570 (2013),
Application(s): ELISA using MCF7 cell culture supernatants,
Abstract;
Full Text
Lipocalin-2 deficiency attenuates insulin resistance associated with aging and obesity: I.K. Law, et al.; Diabetes
59, 872 (2010),
Application(s): EIA using mouse tissue homogenates,
Abstract;
Full Text
Role of 12/15-lipoxygenase in nitrosative stress and peripheral prediabetic and diabetic neuropathies: R. Stavniichuk, et al.; Free Radic. Biol. Med.
49, 1036 (2010),
Application(s): EIA using mouse tissue homogenates,
Abstract;
Effects of vitamin E on mitochondrial dysfunction and asthma features in an experimental allergic murine model: U. Mabalirajan, et al.; J. Appl. Physiol.
107, 1285 (2009),
Application(s): EIA using mouse tissue homogenates,
Abstract;
Full Text
Foxp3 regulates megakaryopoiesis and platelet function: J.J. Bernard, et al.; Arterioscler. Thromb. Vasc. Biol.
29, 1874 (2009),
Application(s): EIA using mouse serum and plasma,
Abstract;
Full Text
5-LOX, 12-LOX and 15-LOX in immature forms of human leukemic blasts: Y. Denizot, et al. ; Leuk. Res.
32, 1756 (2008),
Application(s): EIA using human culture supernatants,
Abstract;
Regulation of renal 12(S)-hydroxyeicosatetraenoic acid in diabetes by angiotensin AT1 and AT2 receptors: E.M. Abdel-Rahman, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol.
295, R1473 (2008),
Application(s): EIA using mouse renal interstitial fluid,
Abstract;
Full Text
General Literature References
Oral green tea catechin metabolites are incorporated into human skin and protect against UV radiation-induced cutaneous inflammation in association with reduced production of pro-inflammatory eicosanoid 12-hydroxyeicosatetraenoic acid: L.E. Rhodes, et al.; Br. J. Nutr.
28, 1 (2013),
Abstract;
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