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Sensitive – optional acetylation protocol increases sensitivity 10-fold (0.088 pmol/ml)
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Rapid – reliable results in just 3 hours
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Trusted – regularly cited in peer-reviewed publications
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Reduce errors – ready-to-use color-coded reagents and pre-coated plates
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Simple – efficient and well-established sample handling protocols
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Reliable – consistent lot-to-lot performance
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Save with bulk kit package!
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.
Product Details
| Alternative Name: | cyclic GMP |
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| Sensitivity: | (non-acetylated) 0.37 pmol/ml; (acetylated) 0.088 pmol/ml (range (non-acetylated) 0.16 - 500 pmol/ml; (acetylated) 0.16 - 100 pmol/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of cGMP in culture supernatants, plasma, serum, saliva, and urine from any species. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Crossreactivity: | cyclic GMP (100%) and <0.001%: GMP, GTP, cyclic AMP, AMP, ATP, cyclic UMP, CTP |
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| Use/Stability: | Store all components at +4°C, except standard and conjugate at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | GxR Microtiter plate, Conjugate, Antibody, Assay buffer 2 concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine |
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| Scientific Background: | Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Effects of a Soluble Guanylate Cyclase Stimulator Riociguat on Contractility of Isolated Pulmonary Artery and Hemodynamics of U46619-Induced Pulmonary Hypertension in Dogs: S. Kameshima, et al.; Vet. Sci.
10, 159 (2023),
Abstract;
A Series of Substituted Bis-Aminotriazines Are Activators of the Natriuretic Peptide Receptor C: R.J. Smith, et al.; J. Med. Chem.
65, 5495 (2022),
Abstract;
Double-blind controlled dietary cross-over intervention with differentially fertilised intact lettuce leaves shows acute reduction in blood pressure in young adults, associated with faster uptake of nitrate than of phenolics: O.K. Qadir, et al.; Eur. J. Nutr.
61, 4191 (2022),
Abstract;
Evaluation of Multivalent Enterotoxigenic Escherichia coli Vaccine Candidate MecVax Antigen Dose-Dependent Effect in a Murine Model: H. Seo, et al.; Appl. Environ. Microbiol.
88, e0095922 (2022),
Abstract;
Vaccine Candidate Double Mutant Variants of Enterotoxigenic Escherichia coli Heat-Stable Toxin: E.D. Zegeye, et al.; Vaccines
10, 241 (2022),
Abstract;
Discovery of small molecule guanylyl cyclase A receptor positive allosteric modulators: S.J. Sangarlingham, et al.; PNAS
118, e210938611 (2021),
Abstract;
The phosphodiesterase 5 inhibitor sildenafil decreases the proinflammatory chemokine IL-8 in diabetic cardiomyopathy: in vivo and in vitro evidence: S. Giannattasio, et al.; J. Endocrinol. Invest.
42, 715 (2019),
Abstract;
Full Text
Tyrosine kinase inhibitors relax pulmonary arteries in human and murine precision-cut lung slices: A.D. Rieg, et al.; Respir. Res.
20, 111 (2019),
Application(s): Lysates of human pulmonary arteries and veins,
Abstract;
Full Text
Neprilysin inhibition and endothelin-1 elevation: Focus on the kidney: L.C.W. Roksnoer, et al.; Eur. J. Pharmacol.
824, 128 (2018),
Abstract;
Resveratrol treatment may preserve the erectile function after radiotherapy by restoring antioxidant defence mechanisms, SIRT1 and NOS protein expressions: T.E. Sener, et al.; Int. J. Impot. Res.
30, 179 (2018),
Application(s): Rat cavernosal tissue lysate,
Abstract;
Guanylin and uroguanylin mRNA expression is increased following Roux-en-Y gastric bypass, but guanylins do not play a significant role in body weight regulation and glycemic control: M.L. Fernandez-Cachon, et al.; Peptides
101, 32 (2017),
Abstract;
Sildenafil attenuates hypoxic pulmonary remodelling by inhibiting bone marrow progenitor cells: S. Favre, et al.; J. Cell. Mol. Med.
21, 871 (2017),
Abstract;
A Soluble Guanylate Cyclase Activator Inhibits the Progression of Diabetic Nephropathy in the ZSF1 rat: C.M. Boustany-Kari, et al.; J. Pharmacol. Exp. Ther.
356, 712 (2016),
Application(s): Cyclic GMP levels were determined in frozen kidney cortex sections,
Abstract;
Full Text
Ginsenoside Re Enriched Fraction (GS-F3K1) from Ginseng Berries Ameliorates Ethanol-Induced Erectile Dysfunction via Nitric Oxide-cGMP Pathway: M.K. Pyo, et al.; Nat. Prod. Sci.
22, 46 (2016),
Application(s): Measured the cGMP level in the corpus cavernosum tissue,
Full Text
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. 21, 228 (2016), Application(s): Measured rat serum cGMP levels,
Platelet inhibitory effects of juices from Pachyrhizus erosus L. root and Psidium guajava L. fruit: a randomized controlled trial in healthy volunteers: T. Thaptimthong, et al.; BMC Complement. Altern. Med.
16, 269 (2016),
Application(s): Determination of plasma cGMP concentrations,
Abstract;
Full Text
Quercetin Inhibits Pacemaker Potentials via Nitric Oxide/cGMP-Dependent Activation and TRPM7/ANO1 Channels in Cultured Interstitial Cells of Cajal from Mouse Small Intestine: H. Gim, et al.; Cell. Physiol. Biochem.
35, 2422 (2015),
Application(s): ELISA using murine small intestine interstitial cells of Cajal (ICCs),
Abstract;
Full Text
The Influence of Nitric Oxide on Soluble Guanylate Cyclase Regulation by Nucleotides: the Role of Pseudosymmetric Site: N. B. Surmeli, et al.; J. Biol. Chem.
290, 15570 (2015),
Application(s): ELISA,
Abstract;
Full Text
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