United States
Assay Kits, Enzymes, and Substrates for HTS
Enzyme Activity Assays
An extensive range of assay kits, enzymes, and substrates is available to assist with primary and secondary screening. HTS-friendly, off-the-shelf assays are supplied with enzyme, substrate and detection reagents ready for straightforward evaluation by your assay development team. Active, quality-controlled enzymes and sensitive substrates are available for important drug discovery targets including:
FluoForte™ Calcium Assays for GPCR & Calcium Channel Screening
Based upon a new fluorescent calcium indicator, FluoForte™ calcium assay kits offer superior calcium mobilization assays for screening GPCRs and calcium channels. FluoForte™ is more than 2-fold brighter than Fluo-4, yields more robust signal (Z’-factor ≥ 0.75) and provides a larger assay window; making it suitable for challenging cell lines or receptors and increasing the probability of detecting weak agonists.
- Homogeneous mix-and-read, no-wash calcium mobilization assay
- Load dye in live cells at 37°C or even room temperature for easier automation
- Provides comparable EC50 values to commercially available calcium assays
- No quencher dye - no interference from compound ligand-receptor interactions
ProteoStat™ Thermal Shift Assay
Determining the optimal conditions that prevent aggregation of protein targets during storage and handling is critical to a successful high-throughput screen. In addition, determining that hits from screening are due to inhibition and not aggregation effects is an important secondary screen in the hit-to-lead process. The ProteoStat™ Thermal Shift Stability Assay is a rapid, simple fluorescent screening method that detects protein aggregates.
- Determines the impact of ligand-protein interactions on target protein stability
- Accelerated analysis of parameters impacting monoclonal antibody and recombinant protein stability
- Measures aggregation temperature
