| HeLa cells were stained with CYTO-ID® Green Detection Reagent 2 after being cultured in (A) full media or (B) starvation media (EBSS) with 40µM Chloroquine for 4h. Cells starved in EBSS in the presence of Chloroquine showed very bright green fluorescent signals and punctate structures. |
CYTO-ID® Autophagy Detection Kit 2.0 measures autophagic vacuoles and monitors autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles. The dye has been optimized through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation into pre-autophagosomes, autophagosomes, and autolysosomes (autophagolysosomes). The assay offers a rapid and quantitative approach to monitoring autophagy in live cells without the need for cell transfection.
We offer a no-transfection assay which eliminates the need for transfection efficiency validation. Using a proprietary, non-covalent, cell labeling technology, CYTO-ID® Autophagy Detection Kit 2.0 provides a method for quantifying and detecting autophagy in live cell formats, offering a convenient solution for live cell analysis. Our kit offers a brighter, more photostable dye which stains autophagic vesicles, ensuring low background while guaranteeing specific staining.
Enzo Life Sciences is a leader in labeling and detection with over 40 years of manufacturing experience for the research market.
Like many other Enzo Life Sciences’ CELLESTIAL® Cellular Analysis products, the CYTO-ID® Autophagy Detection Kit 2.0 is supplied in an easy-to-use, complete kit format.
For more information on, or to order, the CYTO-ID® Autophagy Detection Kit 2.0 or other Enzo Life Sciences products, please visit us at www.enzolifesciences.com or call us at 1-800-942-0430.
Enzo Life Sciences, Inc. is a wholly owned subsidiary of Enzo Biochem, Inc. headquartered in Farmingdale, New York. Enzo Biochem is a pioneer in molecular diagnostics, leading the convergence of clinical laboratories, life sciences and therapeutics through the development of unique diagnostic platform technologies. A global company, Enzo Biochem utilizes cross-functional teams to develop and deploy products and services that meet the ever-changing and rapidly growing needs of healthcare. Underpinning Enzo Biochem’s technologies, platforms and products is a broad and deep intellectual property portfolio, with patent coverage across a number of key enabling technologies.
Enzo Life Sciences, Inc. is a pioneer in labeling and detection technologies from genome to whole cell across research and diagnostic markets. We are focused on providing cost-effective, relevant and reliable solutions for life science research, drug development, and clinical research. We are organized to lead in the development, production, marketing, and sales of innovative research tools worldwide based on 40 years’ experience in building strong global market recognition and implementing outstanding operational capabilities.
New Product Release
Date: May 19th 2016
For additional Information, please contact:
Kara Cannon, Global Head of Marketing
1-631-694-7070 x 1301
