Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Three Dimensional Hepatotoxicity Screening using Corning® HepatoCells, the Spheroid Microplate, and the SCREEN-WELL® Hepatotoxicity Library

Albert Wong1, Jonathan Weinreich1, Alexandra Wolff1, Hilary Sherman2, Hannah J. Gitschier2, and David H. Randle Ph.D2
1 Enzo Life Sciences, Farmingdale, NY, USA
2 Corning Incorporated, Life Sciences, Kennebunk, Maine USA

Featured Product: SCREEN-WELL® Hepatotoxicity Library

> Download the complete application note


Abstract

Having the right model for drug screening is essential for predicting which compounds may cause drug-induced liver injury. Three dimensional (3D) models offer significant improvements over traditional two dimensional monolayer cell culture in terms of maintaining morphological and functional characteristics of tissue, and may provide a better representation of in vitro drug toxicity1. Here we demonstrate how Corning® HepatoCells, an immortalized alternative to primary human hepatocytes, in conjunction with Corning spheroid microplates can be utilized for a 3D drug screen to discover potential hepatotoxins. Hepatospheres formed using Corning HepatoCells were compared to spheroids formed using alternative immortalized hepatocyte cells. Cell viability, urea, and albumin were used to assess hepatotoxicity of the hepatospheres after exposure to the SCREEN-WELL® Hepatotoxicity Library from Enzo Life Sciences, a library consisting of 238 compounds with a variety of structurally and mechanistically different compound classes, as well as nontoxic controls. Selected hits identified in the 3D screen, which significantly reduced cell viability of the hepatospheres, were then assessed in a dose-dependent manner for potency analysis. These results demonstrate that Corning HepatoCells, together with Corning spheroid microplates, are powerful tools that can be used for reliable and reproducible 3D hepatotoxicity screening.

Read More about Toxicology

TechNotes

Our bi-weekly TechNotes are an easy way for you to keep in touch with how Enzo Life Sciences is living up to our motto: Scientists Enabling Scientists™. Each TechNote connects recently published research with one or more of the many solutions Enzo provides for scientists across the life sciences, drug development, and clinical research communities.

Toxicology TechNotes

Toxicology Product Range

Innovative Solutions for Early Safety Assessment.

Predictive Toxicology

 

Recommend this page

 
Keep in touch

©2017 Enzo Life Sciences, Inc.,