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Compatibility of POLYVIEW® IHC Detection Reagents and HIGHDEF® IHC Chromogens with Automated IHC Slide Stainers

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Colin Nixon1, Morgan Mathieu2, and Wini Luty3
1Beatson Institute for Cancer Research, Glasgow, UK
2Enzo Life Sciences, Lausen, Switzerland
3Enzo Life Sciences, Farmingdale, New York, USA


Featured Products: POLYVIEW® IHC reagent (mouse‐HRP), POLYVIEW® IHC reagent (rabbit‐HRP), HIGHDEF® IHC chromogen substrate (DAB, stable)

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ABSTRACT

Immunohistochemistry is an important diagnostic, prognostic and research tool to analyze tissues of different origins and different species. However, IHC reagents are not always easily adapted to highthroughput staining methods. This application note demonstrated the use of Enzo Life Sciences’ POLYVIEW® IHC detection reagents and HIGHDEF® IHC chromogen with Dako Autostainer Link 48 and Biocare Medical intelliPATH™ FLX as platforms to stain key biomarkers used in both fundamental and clinical cancer research.

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