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IL-15 (human), (recombinant)

 
ENZ-PRT255-0100 100 µg 862.00 USD
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Product Specification

Alternative Name:Interleukin-15, IL-T
 
MW:~12.9kDa
 
Source:Produced in E. coli. Contains 115 amino acids.
 
UniProt ID:P40933
 
Formulation:Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium bicarbonate, pH 8.
 
Purity:≥95% (SDS-PAGE)
 
Endotoxin Content:≤1 EU/µg protein (LAL test)
 
Reconstitution:Reconstitute in sterile water at 0.1 mg/mL.
 
Shipping:Ambient
 
Long Term Storage:-80°C
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. For long term storage, it is recommended to dilute to working aliquots in a 0.1% BSA solution.
 
Scientific Background:Interleukin 15 (IL-15) is a widely expressed proinflammatory cytokine that is structurally and functionally related to interleukin 2 (IL-2). IL-15 signals through JAK kinases to activate STAT3, STAT5, and STAT6 transcription factors. IL-15 regulates the activation of T cells, neutrophils, and macrophages, and is a stimulatory cytokine promoting dendritic cell function. IL-15 expression is dysregulated in chronic inflammatory disorders, including rheumatoid arthritis, psoriasis, and pulmonary inflammatory diseases.
 
ENZ-PRT255
Figure 1: Serial dilutions of Human IL-15 (starting at 1 ng/mL) were added to CTLL-2 cells with other needed cytokines and stimuli. After 65 hours, cell viability was measured and the linear portion of the curve can be used to calculate the ED50.
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