| Alternative Name: | Interleukin-21 |
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| MW: | ~15.1 kDa |
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| Source: | Produced in E. coli. Contains 130 amino acids. |
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| UniProt ID: | Q9ES17 |
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| Formulation: | Lyophilized from a sterile (0.2 micron) filtered solution containing 20 mM sodium bicarbonate, pH 8.5. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Endotoxin Content: | ≤1 EU/µg protein (LAL test) |
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| Biological Activity: | Mouse thymocyte proliferation with costimulation; ED50 ≤ 10 ng/mL (≥ 1.0 x 105 units/mg). |
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| Reconstitution: | Reconstitute in sterile water at 0.1 mg/mL. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. For long term storage, it is recommended to dilute to working aliquots in a 0.1% BSA solution. |
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| Scientific Background: | Interleukin 21 (IL-21) is normally expressed by activated CD4+ T cells. The IL-21 receptor activates the JAK/STAT signaling pathway and is expressed on T, B, and natural killer (NK) cells. IL-21 cooperates with IL-4 for the production of multiple antibody classes in B cells. IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, NK, and dendritic cells. |
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| Regulatory Status: | RUO - Research Use Only |
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