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IL-21 (human), (recombinant)

ENZ-PRT248-0010 10 µg 268.00 USD
ENZ-PRT248-0100 100 µg 1,456.00 USD
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Product Specification

Alternative Name:Interleukin-21
MW:~15.4 kDa
Source:Produced in E. coli. Contains 132 amino acids.
UniProt ID:Q9HBE4
Formulation:Lyophilized from a sterile (0.2 micron) filtered solution containing 10 mM sodium phosphate, pH 7.5.
Purity:≥95% (SDS-PAGE)
Endotoxin Content:≤1 EU/µg protein (LAL test)
Biological Activity:B9 cell proliferation; ED50 ≤ 50 ng/mL (≥ 2.0 x 104 units/mg).
Reconstitution:Reconstitute in sterile water at 0.1 mg/mL.
Long Term Storage:-80°C
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C. For long term storage, it is recommended to dilute to working aliquots in a 0.1% BSA solution.
Scientific Background:Interleukin 21 (IL-21) is normally expressed by activated CD4+ T cells. The IL-21 receptor activates the JAK/STAT signaling pathway and is expressed on T, B, and natural killer (NK) cells. IL-21 cooperates with IL-4 for the production of multiple antibody classes in B cells. IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, NK, and dendritic cells.
Figure 1: Serial dilutions of Human IL-21 (starting at 30 ng/mL) were added to ANBL-6 cells. After 84 hours, cell proliferation was measured and the linear portion of the curve can be used to calculate the ED50.
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