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PD-L1 (human), (recombinant)

 
ENZ-PRT223-0100 100 µg 350.00 USD
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Product Specification

Alternative Name:Programmed cell death 1 ligand 1, CD274, B7-H1
 
MW:~27kDa (actual is a doublet at 45kDa and 50kDa)
 
Source:Produced in HEK cells. Human PD-L1 (aa 19-239) is fused at the N-terminus to a His-tag and FLAG®-tag.
 
UniProt ID:Q9NZQ7
 
Concentration:0.5 mg/mL after reconstitution.
 
Formulation:Lyophilized. Contains PBS.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<0.1 EU/μG (LAL test, Cape Cod Associates)
 
Reconstitution:Reconstitute in 200µl PBS.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:-20°C
 
Handling:Spin tube prior to reconstitution. Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Scientific Background:PD-L1, also known as B7-H1 and CD274, is a 290 amino acid transmembrane glycoprotein in the B7 family of immune regulatory molecules. It has an extracellular domain that can interact with PD-1, which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. The PD1/PD-L1 interaction ensures that activation of the immune system occurs at the appropriate time. This will minimize the possibility of chronic autoimmune inflammation.  PD-L1 is known to inhibit proliferation of T cells via apoptosis. It also plays and important role in arresting cell-cycle progression. This makes PD-L1 an attractive therapeutic target for cancer and immunologic diseases.
 
ENZ-PRT223 PD-1-Activity-Fig3
PD-L1 (Prod. No. ENZ-PRT223) was coated onto high binding plates at 10ug/mL. PD-1:human Fc was titered into the assay. The reactions were incubated for 1 hour at room temperature. The plates were washed 3X. Goat anti human IgG-HRP was added into the wells and the plate was incubated for 1 hour then washed 3X. TMB substrate was added. The reaction was stopped with 1M HCL and the plate was read at OD450nm. PD-1 binding to PD-L1 is in the range of 10 – 10,000 ng/ml.
ENZ-PRT223 PD-L1-Blots-Fig2
Western Blot Anaysis. Lane 1,3 and 6, Molecular weight marker. 100ng PD-L1 (Prod. No. ENZ-PRT223) Probed with (Lane 2) mouse anti-FLAG monoclonal antibody, (Lane 4) mouse anti-HIS monoclonal antibody, and (Lane 6) mouse anti-PD-L1 monoclonal antibody.
ENZ-PRT223 PD-1-Fig1 SDS-PAGE
Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0 µg PD-L1. Lane 3, 1.0 µg PD-L1.
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ENZ-PRT223 PD-1-Activity-Fig3 ENZ-PRT223 PD-L1-Blots-Fig2 ENZ-PRT223 PD-1-Fig1 SDS-PAGE

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