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Prolactin (human), (recombinant)

 
ENZ-PRT194-0050 50 µg 255.00 USD
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Product Specification

Alternative Name:Luteotropic hormone, Luteotropin
 
MW:Expected 24.6kDa, actual is doublet at ~35kDa and ~30kDa.
 
Source:Produced in HEK 293 cells. Prolactin (aa 29-227) is fused at the N-terminus to a FLAG-tag, 13 amino acid spacer, and a His-tag.
 
UniProt ID:P01236
 
Formulation:Lyophilized from 1X PBS, pH 7.5.
 
Purity:≥90% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:≤100EU/mg
 
Biological Activity:Positive for NB2-11 cell proliferation.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:After reconstitution, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
 
Scientific Background:Prolactin is a hormone, produced by the anterior pituitary gland, that has been shown to induce lactation in the mammary glands after birth. It has since been shown to have many diverse regulatory functions in the body including reproductive, metabolic, osmotic, immunological, and behavioral roles. Elevated levels of prolactin can be associated with hypothalamic disease, cancer, hypothyroidism, and kidney disease. Prolactin levels can be controlled inhibitory factors, such as dopamine. Prolactin has also been shown to stimulate proliferation of Nb2-11cells in a dose dependent manner.
 
ENZ-PRT194 Proliferation Assay
Nb2-11 cell proliferation assay after treatment with prolactin (ENZ-PRT194-0050): Nb2-11 cells were placed in stationary media at 50,000cells/well in 96 well plates. They were treated with a titered concentration of prolactin. The cells were grown at 37OC / 5% CO2 for 48 hours. Proliferation was measured using an MTT cell viability assay. The ED50 range is 30 - 200pg/ml.
ENZ-PRT194 SDS-PAGE
Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg prolactin (ENZ-PRT194-0050).
ENZ-PRT194 Western Blot
Western blot analysis of prolactin probed with rabbit anti FLAG antibody: Lane 1: MWM, Lane 2: 100ng prolactin (ENZ-PRT194-0010) probed FLAG antibody
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ENZ-PRT194 Proliferation Assay ENZ-PRT194 SDS-PAGE ENZ-PRT194 Western Blot

General Literature References

Minireview: extrapituitary prolactin: an update on the distribution, regulation, and functions: R.J. Marano, et al. ; Mol. Endocrinol. 28, 622 (2014), Abstract;
Dopamine inhibits basal prolactin release in pituitary lactotrophs through pertussis toxin-sensitive and insensitive signaling pathways: A.E. Gonzalez-Iglesias, et al. ; Endocrinology 149, 1470 (2008), Abstract; Full Text
Prolactin-stimulated growth of cell cultures established from malignant Nb rat lymphomas: P.W. Gout, et al.; Cancer Res. 40, 2433 (1980), Abstract; Full Text
Studies of prolactin secretion in human pregnancy: J.E. Tyson, et al.; Am. J. Obstet. Gynecol. 113, 14 (1972), Abstract;

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For Research Use Only. Not for use in diagnostic procedures.
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