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IFN-α-2b (human), (recombinant) (active)

Active enzyme with antiviral and antiproliferative properties.
 
ENZ-PRT192-0010 10 µg 214.00 USD
 
ENZ-PRT192-5010 5x10 µg 857.00 USD
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Product Specification

Alternative Name:Interferon-α-2b
 
MW:~21kDa
 
Source:Produced in HEK cells. The human cytokine (aa 24-188) is fused at the N-terminus to a FLAG®-tag followed by an 8 AA linker.
 
UniProt ID:P01563
 
Concentration:0.1 mg/ml when reconstituted as suggested.
 
Formulation:Lyophilized. In PBS.
 
Purity:≥90% (SDS-PAGE)
 
Purity Detail:Affinity purified.
 
Endotoxin Content:<0.1EU/µg protein (LAL test)
 
Activity:>5000 IU/µg
 
Reconstitution:Reconstitute with 100µl sterile distilled water. 
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Scientific Background:IFN-α-2 is a cytokine that displays antiviral and antiproliferative properties. It is comprised of three major subtypes, which include IFN-α-2a, IFN-α-2b, and IFN-α-2c. IFN-α-2b is the predominant subtype in the human genome. It binds to its receptor and activates the JAK-STAT pathway, which stimulates expression of the host genes responsible for antiviral activity. IFN-α-2b is used to treat many diseases, such as hepatitis B and C, melanoma, Kaposi's sarcoma, chronic myeloid lymphoma, and angioblastoma. The combination of IFN-α-2b with ribavirin, a viral inhibitor, has become the standard treatment for chronic hepatitis C virus infection.
 
Technical Info/Product Notes:FLAG® is a registered trademark of Sigma-Aldrich Co.
 
ENZ-PRT192 SDSPage
Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0µg Human IFN-α-2b (ENZ-PRT192).
ENZ-PRT192 Analysis
ENZ-PRT192 activity was determined by comparing against the WHO International Standard for Interferon Alpha 2b (NIBSC, Code 95/566) using the HEK-Blue™ hTLR5 reporter cell line (Invivogen). Briefly, cells were plated at 50,000 cells per well in 100μl of test media. Known amounts of WHO standard and serially diluted ENZ-PRT192 were added in a volume of 100μl. The cells were grown for 20-24 hours at 37°C + 5% CO2. After incubation, 180μl of Quanti-Blue™ was added to 20μl of HEK-Blue™ hTLR5 reporter cell supernatant and the color was allowed to develop for 3 hours at 37°C + 5% CO2. Activity was determined by interpolating the OD at 650nm of the ENZ-PRT192 titer off of the WHO standard curve.
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ENZ-PRT192 SDSPage ENZ-PRT192 Analysis

General Literature References

Heterologous expression, immunochemical and computational analysis of recombinant human interferon alpha 2b: I. Gull, et al.; SpringerPlus 2, 264 (2013), Abstract; Full Text
Variants in interferon-α pathway genes and response to pegylated-interferon-α2a plus ribavirin for treatment of chronic HCV infection in the HALT-C trial: T.M. Welzel, et al.; Hepatology 49, 1847 (2009), Abstract; Full Text
Mechanisms of action of interferon and ribavirin in chronic hepatitis C: Summary of a workshop: R.T. Chung, et al. ; Hepatology 47, 306 (2008), Full Text
Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells: M. Loignon, et al.; BMC Biotechnology 8, 65 (2008), Abstract; Full Text
Identification of nine interferon-α subtypes produced by Sendai virus-induced human peripheral blood leucocytes: T.A. Nyman, et al.; Biochem. J. 329, 295 (1998), Abstract; Full Text

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