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PD-1 (human), (recombinant)

 
ENZ-PRT190-0050 50 µg 301.00 USD
 

Product Specification

Alternative Name:Programmed cell death-1, CD279
 
MW:~20.5kDa (actual is a doublet at 40kDa and 50kDa that is immunoactive with PD-1)
 
Source:Produced in HEK cells. Extracellular domain of PD-1 (aa 25-167) containing a 5’-His-tag, V5 epitope tag spacer, and a FLAG-tag.
 
UniProt ID:Q15116
 
Formulation:Lyophilized.
 
Purity:≥90% (SDS-PAGE)
 
Endotoxin Content:<100 EU/mg 
 
Biological Activity:Binds to PD-L1.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Scientific Background:PD-1 (programmed cell death-1) is a transmembrane protein important for regulating immune responses. It is a member of the CD28/CTLA-4 family of immunoreceptors and is expressed on T cells and pro-B cells suggesting it has a broad spectrum of immune regulation. PD-1 along with its two ligands, PD-L1 and PD-L2, prevent the activation of T cells and protect tissues from autoimmune attack. PD-1 and its ligands are also involved in reduction of infectious immunity, as well as tumor immunity. PD-1 has been shown to facilitate chronic infection and tumor progression. Drugs targeting PD-1 have shown promise in treating cancer and HIV and may be important in a variety of immune related disorders.
 
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
 
ENZ-PRT190 activity
PD-1 Activity. PD-1 activity is in the range of 100 – 25,000 ng/ml. This was done by measuring PD-1’s ability to bind to human PD-L1 (B7-H1) that was immobilized onto magnetic beads. Briefly, PD-1 was titered into the assay, which contained PD-L1 coated magnetic beads and goat anti – human PD-1 conjugated to phycoerythrin (PE). The reactions were incubated for 1 hour at room temperature, washed 3X with PBS, and resuspended in PBS. Each reaction was analyzed by measuring median PE intensity on a BD FacsCalibur cytometer.
ENZ-PRT190 anti Flag WB
Western Blot Analysis. Lane 1, Molecular weight marker. Lane 2, 100ng PD-1. Probed with rabbit anti-FLAG polyclonal antibody.
ENZ-PRT190 anti PD WB
Western Blot Analysis. Lane 1, Molecular weight marker. Lane 2, 100ng PD-1. Probed with mouse anti-PD-1 monoclonal antibody.
ENZ-PRT190 SDS-PAGE
Coomassie stained SDS-PAGE. Lane 1, Molecular weight marker. Lane 2, 1.0 µg PD-1.
Please mouse over
ENZ-PRT190 activity ENZ-PRT190 anti Flag WB ENZ-PRT190 anti PD WB ENZ-PRT190 SDS-PAGE

General Literature References

PD-1 pathway inhibitors: changing the landscape of cancer immunotherapy: D.E. Dolan, et al.; Cancer Control 21, 231 (2014), Abstract; Full Text
Programmed death-1-induced interleukin-10 production by monocytes impairs CD4+ T cell activation during HIV infection: E.A. Said, et al.; Nat. Med. 16, 452 (2010), Abstract; Full Text
The PD-1 pathway in tolerance and autoimmunity: L.M. Francisco, et al.; Immunol. Rev. 236, 219 (2010), Abstract; Full Text
CTLA-4 and PD-1 receptors inhibit T-cell activation by distinct mechanisms: R.V. Parry, et al.; Mol. Cell. Biol. 25, 9543 (2005), Abstract; Full Text
Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade: Y. Iwai, et al. ; PNAS 99, 12293 (2002), Abstract; Full Text
Engagement of the Pd-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation: J.F. Gordon, et al.; J. Exp. Med. 192, 1027 (2000), Abstract; Full Text

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