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Shrimp alkaline phosphatase, (recombinant)

Heat-labile alkaline phosphatase for dephosphorylation of DNA, RNA and nucleotides
 
ENZ-PRT161-1000 1000 U 125.00 USD
 
ENZ-PRT161-5000 5000 U 504.00 USD
Do you need bulk/larger quantities?
 
  • Very high specific activity
  • Completely heat-inactivated at 65°C
  • Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
  • Works in restriction enzyme buffers
  • Used in PCR clean-up to treat dNTPs prior to DNA sequencing or SNP analysis
Shrimp alkaline phosphatase (recombinant), (rSAP) is a multipurpose alkaline phosphatase that is fully inactivated by a short heat treatment. rSAP has all the properties of the native enzyme, but with the additional benefits of greater stability, consistency, and purity.

rSAP simplifies cloning and sequencing workflows due to a complete heat inactivation.

Product Specification

Alternative Name:rSAP
 
MW:54 kDa
 
Source:Pandalus borealis (Northern red shrimp) origin, recombinantly produced in Pichia pastoris.
 
UniProt ID:Q9BHT8_PANBO
 
Concentration:1U/µl for 1000 unit size
5U/µl for 5000 unit size
 
Formulation:Liquid.
 
Purity Detail:Purified by SDS-PAGE. DNase, RNase or protease activities not detected.
 
Specific Activity:>2000 units/mg. One unit of Shrimp alkaline phosphatase (recombinant) converts 1µmol p-nitrophenyl phosphate per minute to nitrophenol and phosphate at 37°C and pH 10.4 in 0.1 M glycine buffer, 1 mM each of ZnCl2 and MgCl2 and 6 mM 4-nitrophenyl phosphate.
 
Applications:Cloning
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Use/Stability:Stable for 2 years at -20°C, for 6 months at 4°C, or for 3 months at 25°C
 
ENZ-PRT161 Fig1
Fig 1. Heat inactivation of Shrimp alkaline phosphatase at 65°C and 75°C
ENZ-PRT161 Fig2
Fig 2. Stability of Shrimp alkaline phosphatase at room temperature
ENZ-PRT161 workflow clone
Fig 3. Cloning workflow using Shrimp alkaline phosphatase (SAP)
ENZ-PRT161 workflow seq
Fig 4. Sequencing workflow using Shrimp alkaline phosphatase (SAP)
Please mouse over
ENZ-PRT161 Fig1 ENZ-PRT161 Fig2 ENZ-PRT161 workflow clone ENZ-PRT161 workflow seq

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Alkaline phosphatase
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