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Diaphorase

 
ENZ-PRT155-0500 500 U 153.00 USD
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Product Specification

Alternative Name:NADPH dehydrogenase
 
MW:~56 kDa
 
Source:Produced in E. coli.
 
EC:1.6.99.1
 
CAS:9001-68-7
 
Formulation:Liquid. In 50mM TRIS, pH 8.0, containing 150mM NaCl.
 
Purity:≥95%
 
Purity Detail:Purified by multi-step chromatography.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-80°C
 
Long Term Storage:-80°C
 
Scientific Background:Diaphorase belongs to a ubiquitous class of flavin-bound enzymes that catalyze the reduction of various dyes which act as hydrogen acceptors from the reduced form of di- and tri- phosphopyridine nucleotides (i.e. NADH, NADPH).
 
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General Literature References

A new assay for diaphorase activity in reagent formulations, based on the reduction of thiazolyl blue: R.S. Boethling & T.L. Weaver; Clin. Chem. 25, 2040 (1979), Abstract;
The identity of diaphorase and lipoyl dehydrogenase: V. Massey; Biochim. Biophys. Acta. 37, 314 (1960), Abstract;

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