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Epidermal growth factor (human), (recombinant)

 
ENZ-PRT154-0010 10 µg 201.00 USD
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Product Specification

Alternative Name:EGF, URG, HOMG4
 
MW:~6.3 kDa
 
Source:Produced in HEK 293 cells.
 
UniProt ID:P01133
 
Formulation:Lyophilized from 0.22 µm filtered solution in PBS. Generally 5-8% Mannitol is added as a protectant before lyophilization.
 
Purity:≥97% (SDS-PAGE)
 
Endotoxin Content:<1 EU/µg (LAL test)
 
Applications:ELISA, WB
in vitro Assay
 
Application Notes:Western Blot (control)
 
Reconstitution:Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Use/Stability:The reconstituted solution can be stored at 2-8°C for up to 1 month. For long term storage, store at -20 °C.
 
Handling:Centrifuge vial prior to opening. Do not vortex.
 
Scientific Background:Human epidermal growth factor (EGF, also known as beta-urogastrone) is a 53 amino acid peptide that regulates cell growth, proliferation, and differentiation. EGF is an integral membrane protein that is cleaved and released into the extracellular space where it acts as a hormone. EGF binds cell surface EGF receptors (EGFR), initiating signaling cascades through activation of EGFR tyrosine kinase activity.
 

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