Far-Red 673 dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Far-Red 673 dUTP can also be used for multicolor fluorescence labeling.
This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).
Product Details
Quantity:
Sufficient for approximately 49 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
Concentration:
1mM
Formulation:
Liquid. Solution in water.
Excitation maximum:
673 nm
Emission maximum:
702 nm
Extinction Coefficient:
88,060 M-1 cm-1 (673 nm in water)
Correction Factor (260 nm):
A260/A673= 0.06
Correction Factor (280 nm):
A280/A673= 0.06
Purity:
≥93% (HPLC)
Appearance:
Green liquid.
Applications:
FISH
Shipping:
Dry Ice
Long Term Storage:
-20°C
Handling:
Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.
Regulatory Status:
RUO - Research Use Only
Fluorescence emission profiles of available fluorescently labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
Fluorescence excitation and emission spectra of Far-Red 673 dUTP.
Excitation and emission values, along with extinction coefficients, for Enzo's wide array of fluorescently labeled dUTPs.
