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Digoxigenin-UTP, alkali-stable

Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of RNA probes
 
ENZ-NUC114-0250 250 nmol 210.00 USD
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Digoxigenin-UTP can replace UTP in reactions catalyzed by T3, T7 or SP6 RNA polymerases. The digoxigenin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids. The single-stranded RNA probes offer selectivity unavailable with double-stranded DNA probes, because they are strand-specific. Furthermore, RNA probes hybridize much more efficiently to target molecules than DNA probes because there is no self-hybridization. The hybridized digoxigenin-labeled DNA probes can be detected by their interaction with antibodies coupled to fluorescent dyes or color-producing enzymes.

Product Specification

Alternative Name:Digoxigenin-11-UTP
 
Formula:C43H65N4O22P3
 
MW:1082.92 (free acid)
 
Quantity:25µl
 
Concentration:10mM
 
Formulation:Liquid. Solution in water.
 
Extinction Coefficient:22,600 M-1cm-1 (220nm, pH 7)
 
Purity:≥93% (HPLC)
 
Appearance:Clear, colorless liquid.
 
Applications:ISH (in situ hybridization), Northern Blot
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
ENZ-NUC114 structure
ENZ-NUC114-Packaging
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ENZ-NUC114 structure ENZ-NUC114-Packaging

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