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Digoxigenin-dUTP, alkali-stable

Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of DNA probes
 
ENZ-NUC113-0025 25 nmol 180.00 USD
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Digoxigenin-dUTP replaces dTTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Digoxigenin-dUTP can be used to produce digoxigenin-labeled DNA probes in a variety of labeling reactions including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. The resulting digoxigenin-labeled probe can be used in a variety of hybridization applications incuding Southern blots, Northern blots, or dot blots. The probes can also be used for in situ hybridization procedures on fixed cells and tissues. Digoxigenin-labeled probes have been shown to hybridize to homologous nucleic acid at the same rate and to the same extent as non-labeled probes. The hybridized digoxigenin-labeled DNA probes can be detected by their interaction with antibodies coupled to fluorescent dyes or color-producing enzymes.

This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).

Product Specification

Alternative Name:Digoxigenin-11-dUTP
 
Formula:C43H65N4O21P3
 
MW:1066.92 (free acid)
 
Quantity:Sufficient for approximately 25 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
 
Concentration:1mM
 
Formulation:Liquid. Solution in water.
 
Extinction Coefficient:22,600 M-1cm-1 (220nm, pH 7)
 
Purity:≥93% (HPLC)
 
Appearance:Clear, colorless liquid.
 
Applications:ISH (in situ hybridization), Southern Blot
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Handling:Avoid freeze/thaw cycles.
 
ENZ-NUC113 structure
ENZ-NUC113-Packaging
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ENZ-NUC113 structure ENZ-NUC113-Packaging

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