- More sensitive detection - able to detect lower concentrations of protein
- Easy to use - all reagents are ready-to-use or ready-to-dilute, saving precious time
- Amenable to multiplex analysis - determine source of protein bands with differentiated colors
- Easily adaptable - works with a wide range of mouse-derived proteins
The WESTERNVIEW® Detection Kit (Anti-Mouse) can be used to identify primary antibodies on a Western Blot using immunodetection. The kit contains a complete reagent set, with optimized ready-to-use or ready-to-dilute reagents.
Western Blot of Jurkat cells using WESTERNVIEW™ Detection Kit
Caspase-7, cleaved and uncleaved, run on the WESTERNVIEW™ Detection Kit (Anti-Mouse) (ENZ-KIT182) and a competitor's kit.
Linear dilution of protein (µg) run in the Chromogenic Western Blot Kit (ENZ-KIT182) and the competitor's kit.
Please mouse over
Product Details
| Assay Time: | 2 hours |
| |
| Applications: | WB, Colorimetric detection
|
| |
| Species reactivity: | Mouse
|
| |
| Shipping: | Blue Ice |
| |
| Short Term Storage: | +4°C |
| |
| Long Term Storage: | +4°C |
| |
| Contents: | - Antibody Diluent, 4 x 90 mL
Diluent solution for dilution of primary and secondary antibodies.
- NBT/BCIP, 100 mL
Ready-to-use solution for chromogenic blotting.
- Wash Buffer (10X), 2 x 100 mL
10X Wash Buffer for washing membrane.
- Secondary Antibody (AP Anti-Mouse), 4.5 mL
Anti-Mouse secondary antibody conjugated to alkaline phosphatase for detection of mouse-based primary antibodies in Western Blot.
|
| |
| Scientific Background: | Western Blotting is a common lab technique used to detect proteins in a sample of tissue homogenate or extract. Due to its flexibility, it can be used for a wide range of applications within molecular biology and biochemistry, including detection of post-translational modifications and verification of protein cloning. Using the size and color intensity of the protein band, a semi-quantitative estimation of protein can be derived. |
| |
| Protocol: | WESTERNVIEW® Detection Kit Basic Protocol - Incubate with primary antibody, place the membrane in a plastic tray and wash.
- Incubate with secondary antibody provided in kit.
- Incubate with NBT/BCIP provided for 10 seconds to 2.5 minutes.
- Wash in distilled water and scan the membrane.
For a more detailed protocol, please reference the kit insert. |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
Product Literature References
Mitofusin-2 modulates the epithelial to mesenchymal transition in thyroid cancer progression: M. H. You, et al.; Sci. Rep.
11, 2054 (2021),
Abstract;
Related Products
