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Chromogenic Western Blot Kit (Anti-Mouse)

Chromogenic Western Blot kit with increased signal and sensitivity
 
ENZ-KIT182-0001 1 Kit 349.00 USD
 
  • More sensitive detection - able to detect lower concentrations of protein
  • Easy to use - all reagents are ready-to-use or ready-to-dilute, saving precious time
  • Amenable to multiplex analysis - determine source of protein bands with differentiated colors
  • Easily adaptable - works with a wide range of mouse-derived proteins
The Chromogenic Western Blot Kit (anti-Mouse) can be used to identify primary antibodies on a Western Blot using immunodetection. The kit contains a complete reagent set, with optimized ready-to-use or ready-to-dilute reagents.
ENZ-KIT182 1
ENZ-KIT182 2
PANC-1 cells treated with etoposide. The band at 50 kDa is clearly revealed to be tubulin, while the smaller bands are now known to be cleaved caspase.
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ENZ-KIT182 1 ENZ-KIT182 2

Product Specification

Assay Time:1 hour
 
Applications:WB
 
Species reactivity:Mouse
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Scientific Background:Western Blotting is a common lab technique used to detect proteins in a sample of tissue homogenate or extract. Due to its flexibility, it can be used for a wide range of applications within molecular biology and biochemistry, including detection of post-translational modifications and verification of protein cloning. Using the size and color intensity of the protein band, a semi-quantitative estimation of protein can be  derived.
 
Protocol:Chromogenic Western Blot Kit Basic Protocol
  1. Incubate with primary antibody, place the membrane in a plastic tray and wash.
  2. Incubate with secondary antibody provided in kit.
  3. Incubate with NBT/BCIP provided for 10 seconds to 2.5 minutes.
  4. Wash in distilled water and scan the membrane.
For a more detailed protocol, please reference the kit insert.
 

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