- More sensitive detection - able to detect lower concentrations of protein
- Easy to use - all reagents are ready-to-use or ready-to-dilute, saving precious time
- Amenable to multiplex analysis - determine source of protein bands with differentiated colors
- Easily adaptable - works with a wide range of mouse-derived proteins
The WESTERNVIEW® Dual Detection Kit (Anti-Mouse/Anti-Rabbit) can be used to identify primary antibodies on a Western Blot using immunodetection. The kit contains a complete reagent set, with optimized ready-to-use or ready-to-dilute reagents.
PANC-1 cells treated with etoposide. The band at 50 kDa is clearly revealed to be tubulin, while the smaller bands are known to be cleaved caspase.
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Product Details
| Assay Time: | 2 hours |
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| Applications: | WB
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| Species reactivity: | Mouse
Rabbit
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Contents: |
- Antibody Diluent, 4 x 90 mL
Diluent solution for dilution of primary and secondary antibodies.
- NBT/BCIP, Ready-to-Use, 100 mL
Ready-made solution for chromogenic blotting to be used when developing anti-rabbit secondary.
- DAB Chromogen (25X), 4 mL
Concentrated chromogen for chromogenic blotting to be used when developing anti-mouse secondary.
- DAB Substrate Buffer, 100 mL
Buffer used to dilute DAB Chromogen (25X).
- SignaSure® Buffer Salts, 2 packets
Contents used to make SignaSure® Wash Buffer.
- Tween®-20, 2 mL
Detergent for SignaSure® Wash Buffer.
- Secondary Antibody (HRP Anti-Mouse), 2 mL
Anti-Mouse secondary antibody for detection of mouse-based primary antibodies in Western Blots. Use DAB Substrate Buffer and Chromogen to develop.
- Secondary Antibody (AP Anti-Rabbit), 2 mL
Anti-Rabbit secondary antibody for detection of rabbit-based primary antibodies in Western Blots. Use NBT/BCIP solution to develop.
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| Scientific Background: | Western Blotting is a common lab technique used to detect proteins in a sample of tissue homogenate or extract. Due to its flexibility, it can be used for a wide range of applications within molecular biology and biochemistry, including detection of post-translational modifications and verification of protein cloning. Using the size and color intensity of the protein band, a semi-quantitative estimation of protein can be derived. |
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| Regulatory Status: | RUO - Research Use Only |
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