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PCR decontamination kit

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity
 
ENZ-KIT137-0100 100 Reactions 125.00 USD
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  • Removes DNA contamination from PCR mastermixes
  • Improves target detection by reducing background
  • Does not effect PCR sensitivity
  • Optimized for PCR and probe based qPCR mixes
The PCR decontamination kit uses a dsDNase to remove contaminating DNA from mastermixes. The double-strand specific property allows decontamination in the presence of primers and probe.The dsDNase is irreversibly inactivated by heating to 60°C in the presence of DTT, ensuring that DNA template added to the mastermix after inactivation will not be digested. The mastermix can be used immediately after decontamination to run PCR reactions.

AMPIGENE® Taq polymerases have been treated to remove host DNA and additional clean-up is not necessary. The PCR decontamination kit is recommended for decontamination of primers or competitor’s PCR mixes.
ENZ-KIT137 Fig1
Fig 1. The presence of E. coli 23S DNA in 2x probe master mixes from various suppliers was quantified by using water as template (NTC) and following the manufacturer’s instructions. The figure shows plots acquired from several separate experiments. Traces of E. coli 23S DNA were found in all master mixes tested, with Cq values generally ranging from 30 – 35.
ENZ-KIT137 Fig2
Fig 2. Untreated and decontaminated qPCR 2x master mix was used for analysis of an E. coli gDNA 10-fold serial dilution with 5 steps. NTC samples were included, and all plots of the serial dilution show an average of three replicates. Inset: Standard curves calculated from Cq values obtained from analysis of serial dilution.
ENZ-KIT137 schematic
Fig 3. Simple workflow for decontamination
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ENZ-KIT137 Fig1 ENZ-KIT137 Fig2 ENZ-KIT137 schematic

Product Specification

Application Notes:For the removal of contaminating DNA from primers and mastermixes prior to PCR.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Kit/Set Contains:Heat-labile dsDNase
DTT
 
Scientific Background:Polymerases used in PCR are frequently contaminated with E. coli DNA. Contaminating DNA may cause reduced sensitivity and false positives when small amounts of bacterial DNA are targeted. Other sources of contamination might be dNTPs, buffer components and primers / probes, as well as DNA introduced during handling. The PCR decontamination kit is an easy method to eliminate DNA contamination.
 
UniProt ID:C9YSL6_PANBO
 

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