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gDNA removal kit

Rapid and complete removal of gDNA from RNA preps
 
ENZ-KIT136-0050 50 Reactions 152.00 USD
 
ENZ-KIT136-0250 250 Reactions 609.00 USD
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  • Removes gDNA from RNA prior to reverse transcription in the same tube
  • HL-dsDNase is inactivated without reducing quality or quantity of RNA
  • Minimizes pipetting steps and suitable for high throughput experiments
gDNA removal kit uses a heat labile dsDNase to remove gDNA from RNA preps prior to reverse transcription.

The kit is based on a recombinant heat labile dsDNase which is irreversibly inactivated at low temperatures. This enables an inactivation step including heating to moderate temperatures only (58°C), which is gentle enough to preserve both quality and quantity of all present RNA. Reverse transcription can be performed in the same tube, minimizing pipetting steps and reducing hands-on time.
ENZ-KIT136 Fig1
Fig 1. The gDNA removal kit used in RNA prep prior to RT-qPCR. The gDNA removal kit removes at least 50ng of gDNA in a 10µl reaction volume.
ENZ-KIT136 Fig2
Fig 2. mRNA subjected to the gDNA removal kit protocol. All contaminating gDNA was removed while RNA was left unharmed. Compared to the untreated control, no reduction in quantified cDNA (SDHA) was detected.
ENZ-KIT136 schematic
Fig 3. Simple protocol for removing genomic DNA from RNA
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ENZ-KIT136 Fig1 ENZ-KIT136 Fig2 ENZ-KIT136 schematic

Product Specification

Application Notes:For the removal of gDNA from RNA prior to reverse transcription.
 
Quality Control:Tested for absence of RNase.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-20°C
 
Kit/Set Contains:10x reaction buffer
Heat-labile dsDNase
 
Scientific Background:Most techniques used for RNA isolation yield RNA with significant amounts of contaminating genomic DNA (gDNA), potentially resulting in false and inaccurate mRNA quantification. This is particularly a problem in the quantification of low-copy transcripts or small samples. The gDNA removal kit efficiently removes gDNA from RNA preps to levels below the detection limit of RT-qPCR.
 
UniProt ID:C9YSL6_PANBO
 

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