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Cell migration assay, TC

Reproducibly monitor real-time cell migration without the use of special instrumentation
 
ENZ-KIT113-0001 96 wells 315.00 USD
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  • Membrane-free Migration: perform studies without manipulating transmembrane inserts
  • Reproducible Results: obtain well-to-well CV's <12% due to the unique design
  • Preserves Cell Morphology: monitor changes in cell structure in real-time.
  • Versatile: analyze data using multiple probes in a single well by using a microscope, digital imager, or fluorescence microplate reader.
  • Flexible: perform kinetic or endpoint Cell Migration Assays without the use of special instrumentation.
The Cell migration assay is a reproducible, sensitive, and flexible assay that can be used to monitor cell migration. Formatted for a 96-well plate, the assay utilizes Cell Seeding Stoppers made from a medical-grade silicone to restrict cell seeding to the outer annular regions of the wells. Removal of the stoppers reveals a 2mm diameter unseeded region in the center of each well, i.e., the detection zone, into which the seeded cells may then migrate. The Detection Mask is applied to the plate bottom and restricts visualization to the detection zones, allowing only cells that have migrated to be detected. The Cell migration assay is designed to be used with any stain or labeling technique. Readout can be performed by microscopy or use of a microplate reader.

The Cell migration assay system has been designed for use with adherent cell cultures. This assay has been successfully used with HT-1080, PC-3, A549, NCI H1650, MDA-MB-231, NMuMG, 3T3-Swiss albino, HCEC, HUVEC, and MCF10A cell lines.
ENZ-KIT113 assay formats
Fig 1. Formats of Cell migration assays.
ENZ-KIT113 schematic
Fig 2. Schematic of Cell migration assay.
ENZ-KIT113 migration-green
Fig 3. Cell Migration using CYTO-ID® Green Long-Term Cell Tracer (Prod. No. ENZ-51036) is reproducible. Area of closure (n=3) 70.2±4.1%.
ENZ-KIT113 migration-red
Fig 4. Cell Migration using CYTO-ID® Red Long-Term Cell Tracer (Prod. No. ENZ-51037) is reproducible. Area of closure (n=3) is 71.1±2.4%.
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ENZ-KIT113 assay formats ENZ-KIT113 schematic ENZ-KIT113 migration-green ENZ-KIT113 migration-red

Product Specification

Applications:Fluorescence microscopy, Fluorescent detection
 
Application Notes:2-D assay for investigating cell migration of adherent cell lines
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Kit/Set Contains:Tissue Culture treated 96-well plate (black, clear bottom) with cell seeding stoppers
Cell migration detection mask
Cell migration stopper tool
 

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