Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Cell migration BioGel assay and invasion assay, Collagen-I coated

Reproducibly monitor real-time cell migration or invasion with an automation-friendly design
 
ENZ-KIT110-0001 96 wells 227.00 USD
Do you need bulk/larger quantities?
 
  • Increased Productivity - Treat cells with multiple fluorescent probes, labels, or colorimetric stains for multi-parametric measurements with inverted microscopes, High Content Screening (HCS) and High Content Imaging (HCI) instruments.
  • Less Handling - Realize reduced assay handling time with an assay format in which a centrally placed biocompatible gel automatically dissolves to reveal a detection zone.
  • Automation-Friendly Design - Utilize automated liquid handling equipment for fast set-up of high throughput, 96-well assays.
  • Reproducible Results - Achieve well-to-well CV's ≤12% and generate robust Z’ factors suitable for compound screening.
  • Real-Time Analysis - Experience unlimited access to cells, cell morphology and cell movement throughout your experiment.
The Cell migration BioGel assay and invasion assay is a reproducible, sensitive, and flexible assay that can be used to monitor cell migration. Formatted for a 96-well plate, the assay uses a non-toxic biocompatible gel (BioGel) to form a cell-free zone on cell culture surfaces. After seeding cells into the 96-well plate, the BioGel dissolves permitting cells to migrate into the well centers.

The Cell migration BioGel assay and invasion assay can also be used to monitor cell invasion. After cell seeding, a Collagen-I overlay can be added to create a 3-D extracellular matrix (ECM) for cell invasion. Cell invasion into the Detection Zone can be assessed by performing a Z-stack analysis.

The Cell migration BioGel assay and invasion assay enables the use of automated liquid handling equipment for cell seeding and allows for unlimited access to wells from cell seeding through data readout. The Cell migration BioGel assay and invasion assay is designed to be used with any stain or labeling technique. Researchers can capture and quantify real-time cell migration data using inverted microscopes, High Content Screening (HCS) and High Content Imaging (HCI) instruments.

The Cell migration BioGel assay and invasion assay system has been designed for use with adherent cell cultures. This assay has been successfully used with HT-1080 and MDA-MB-231 cell lines, and Human Umbilical Vein Endothelial Cells (HUVECs).
ENZ-KIT110 assay formats
Fig 1. Formats of Cell migration assays
ENZ-KIT110 incubate plate
Fig 2. BioGel in the Cell migration BioGel assay.
ENZ-KIT110 schematic
Fig 3. Schematic of Cell migration BioGel Assay.
ENZ-KIT110 fluorescence
Fig 4. Cell migration data (n=24) obtained using fluorescence microscopy.
ENZ-KIT110 assay overview
Fig 5. Schematic of Cell migration BioGel Assay and Invasion Assay used with Collagen-I to create overlay.
ENZ-KIT110 cell migration
Fig 6. Cell migration data (n=5 / condition) obtained using BD Pathway™ 855 Bioimaging system.
Please mouse over
ENZ-KIT110 assay formats ENZ-KIT110 incubate plate ENZ-KIT110 schematic ENZ-KIT110 fluorescence ENZ-KIT110 assay overview ENZ-KIT110 cell migration

Product Specification

Applications:Fluorescence microscopy, Fluorescent detection
 
Application Notes:2-D or 3-D assay for investigating cell migration or cell invasion of adherent cell lines
 
Shipping:Ambient
 
Long Term Storage:Ambient
 
Kit/Set Contains:Cell migration BioGel 96-well plate, Collagen-I coated
 

Related Products

Cell migration BioGel assay, TC 

Reproducibly monitor real-time cell migration with an automation-friendly design
Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-KIT109-0001 96 wells 205.00 USD
Do you need bulk/larger quantities?
 

Cell migration BioGel HT assay, TC 

Reproducibly monitor real-time cell migration with a high-throughput platform
Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-KIT111-0001 384 wells 289.00 USD
 
ENZ-KIT111-0005 5x384 wells 1,366.00 USD
Do you need bulk/larger quantities?
 

Cell migration BioGel HT assay, Collagen-I coated 

Reproducibly monitor real-time cell migration with a high-throughput platform
Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-KIT112-0001 384 wells 310.00 USD
 
ENZ-KIT112-0005 5x384 wells 1,419.00 USD
Do you need bulk/larger quantities?
 

Cell migration assay, TC 

Reproducibly monitor real-time cell migration without the use of special instrumentation
Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-KIT113-0001 96 wells 315.00 USD
Do you need bulk/larger quantities?
 

Cell migration assay, Collagen-I coated 

Reproducibly monitor real-time cell migration without the use of special instrumentation
Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-KIT114-0001 96 wells 330.00 USD
Do you need bulk/larger quantities?
 

Cell migration assay, Fibronectin coated 

Reproducibly monitor real-time cell migration without the use of special instrumentation
Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-KIT115-0001 96 wells 353.00 USD
Do you need bulk/larger quantities?
 

Cell migration assay, Tri-Coat 

Reproducibly monitor real-time cell migration without the use of special instrumentation
Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-KIT116-0001 96 wells 367.00 USD
Do you need bulk/larger quantities?
 

Cell migration assay, flex kit 

Reproducibly monitor real-time cell migration without the use of special instrumentation
Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-KIT117-0001 96 wells 335.00 USD
Do you need bulk/larger quantities?
 

Cell migration stopper tool 

Print as PDF
 
ENZ-ACC100-0001 1 ea 32.00 USD
Do you need bulk/larger quantities?
 

Cell migration mask 

Print as PDF
 
ENZ-ACC101-0005 5 Pack 58.00 USD
Do you need bulk/larger quantities?
 

CYTO-ID® Green long-term cell tracer kit 

Live cell fluorescent labeling over extended time periods with no apparent toxic effects
Flow Cytometry | Print as PDF
 
ENZ-51036-K025 1 Kit 204.00 USD
Do you need bulk/larger quantities?
 

CYTO-ID® Red long-term cell tracer kit  

Live  cell fluorescent labeling over extended time periods with no apparent toxic effects
Flow Cytometry, Fluorescence microscopy, Fluorescent detection | Print as PDF
 
ENZ-51037-K025 1 Kit 195.00 USD
Do you need bulk/larger quantities?
 

Collagen I, rat tail 

Isolated from rat tail tendons., ≥90% (SDS-PAGE) | Print as PDF
 
ALX-522-435-0020 20 mg 68.00 USD
 
ALX-522-435-0100 100 mg 157.00 USD
Do you need bulk/larger quantities?
 

Calcein AM (ultra pure) 

Calcium dye
148504-34-1, ≥95% (HPLC) | Print as PDF
 
ENZ-52002 1 mg 135.00 USD
Do you need bulk/larger quantities?
 

DAPI (ultra pure) 

DNA dye
28718-90-3, ≥95% (HPLC) | Print as PDF
 
ENZ-52404 100 mg 562.00 USD
Do you need bulk/larger quantities?
 

E-cadherin (human) monoclonal antibody (SHE78-7) 

Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with soluble E-cadherin from human placenta., ELISA, Flow Cytometry, IF, IHC (FS), IHC (PS), WB, FUNC | Print as PDF
 
ALX-804-203-C100 100 µg 690.00 USD
Do you need bulk/larger quantities?
 

E-cadherin (mouse) monoclonal antibody (ECCD-1) 

Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of Wistar rat immunized with mouse teratocarcinoma cell line F9. Purified from serum-free cell culture supernatant., FUNC | Print as PDF
 
ALX-804-263-C100 100 µg 443.00 USD
Do you need bulk/larger quantities?
 

E-cadherin monoclonal antibody (ECCD-2) 

Obtained by fusing the P3-X63-Ag8 myeloma cell line with splenocytes of Donryu rat immunized with mouse liver E-cadherin fragment., IHC (FS), WB, FUNC | Print as PDF
 
ALX-804-202-C100 100 µg 635.00 USD
Do you need bulk/larger quantities?
 

E-cadherin monoclonal antibody (HECD-1) 

Obtained by fusing the P3-X63-Ag8-U1 myeloma cell line with splenocytes of BALB/c mouse immunized with human breast tumor cell line MCF-7., ELISA, Flow Cytometry, ICC, IHC (FS), IHC (PS), WB, FUNC | Print as PDF
 
ALX-804-201-C100 100 µg 690.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
MSDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Product Flyers
Reproducible Assays for Studying Cell Migration
Reproducible Assays for Studying Cell Migration
Download as PDF

All new literature pieces

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,