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AMPIGENE® cDNA Synthesis Kit

Efficient and sensitive cDNA synthesis for downstream  qPCR
 
ENZ-KIT106-0050 50 Reactions 205.00 USD
 
ENZ-KIT106-0200 200 Reactions 738.00 USD
Do you need bulk/larger quantities?
 
  • Unbiased, efficient, sensitive cDNA synthesis
  • Thermostable and extremely active RTase
  • Enhanced buffer system for efficient synthesis
  • Optimized random hexamer and anchored oligo(dT) concentrations for generation of cDNA for qPCR
AMPIGENE® cDNA Synthesis Kit uses the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient and accurate cDNA synthesis.
The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active.
An optimized buffer system contains oligo(dT), random hexamers, enhancers, dNTPs and MgCl2, allowing efficient and sensitive cDNA synthesis.

Product Specification

Application Notes:For cDNA synthesis for downstream qPCR reactions
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:5x cDNA synthesis mix (anchored oligo (dT), random hexamers, 15mM MgCl2, 5mM dNTPs, enahncers, and stabilizers)
20x RTase (with RNase inhibitor)
 

Product Literature References

Oxidative stress, neurotoxicity, and metallothionein (MT) gene expression in juvenile rock fish Sebastes schlegelii under the different levels of dietary chromium (Cr6+) exposure: J.H. Kim & J.C. Kang; Ecotoxicol. Environ. Saf. 125, 78 (2016), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;
The immune responses and expression of metallothionein (MT) gene and heat shock protein 70 (HSP 70) in juvenile rockfish, Sebastes schlegelii, exposed to waterborne arsenic (As3+): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 47, 136 (2016), Application(s): Specific gene expression - liver samples, Abstract;
The immune responses in juvenile rockfish, Sebastes schlegelii for the stress by the exposure to the dietary lead (II): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 46, 211 (2016), Application(s): Heat shock protein 70 gene expression, liver sample RNA, Abstract;
The toxic effects on the stress and immune responses in juvenile rockfish, Sebastes schlegelii exposed to hexavalent chromium: J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 43, 128 (2016), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;
Growth performance and immunological and antioxidant status of Chinese shrimp, Fennerpenaeus chinensis reared in bio-floc culture system using probiotics: M.S. Kim, et al.; Fish Shellfish Immunol. 47, 141 (2015), Application(s): RNA Reverse transcription, Abstract;
The toxic effects of ammonia exposure on antioxidant and immune responses in Rockfish, Sebastes schlegelii during thermal stress: S.H. Kim, et al.; Environ. Toxicol. Pharmacol. 40, 954 (2015), Application(s): Purified RNA was subjected to reverse transcription to cDNA, Abstract;

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