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Adipogenesis assay kit

Convenient method for the enhanced induction and detection of adipogenesis in the classic 3T3L-1 model.
 
ENZ-KIT103-0005 5x24 wells 260.00 USD
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  • Provides optimized reagents for adipocyte differentiation and subsequent colorimetric lipid oil detection
  • Contains enhancer solution for the optimal induction of adipogenesis
  • For drug-screening and testing agonists/antagonists of adipogenesis
The Adipogenesis Assay Kit provides all the reagents needed to induce and detect adipogenesis. Optimized Differentiation, Insulin, and Enhancer solutions ensure maximal adipocyte induction. The Adipogenesis Dye allows for visual confirmation of differentiation and can also be easily extracted allowing measurement using a spectrophotometer or microplate reader. 
ENZ-KIT103 typical results
ENZ-KIT103 induction
ENZ-KIT103 workflow
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ENZ-KIT103 typical results ENZ-KIT103 induction ENZ-KIT103 workflow

Product Specification

Applications:Colorimetric detection
 
Application Notes:For the induction and colorimetric detection of adipogenesis.
 
Quantity:Contains enough reagents to conduct experiments for:
5 x 24 well plates
or
225 cm2 tissue culture plate surface area
 
Use/Stability:Store Differentiation Solution, Insulin Solution, and Enhancer Solution at -20°C. All other reagents can be stored at room temp.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:1000x Differentiation Solution (Dexamethasone and IBMX)
1000x Insulin Solution 
1000x Enhancer Solution (Rosiglitazone)
1x Cell Fixative
1x Adipogenesis Dye (Oil Red O)
1x Extraction Solution
 
Scientific Background:The ability to regulate the cell cycle and differentiation of adipocytes are key in the development and physiology of obesity. One of the most utilized models for the study of differentiation of fibroblast into adipocytes is the 3T3-L1 cell line. Differentiation of 3T3-L1 cells into adipocytes requires three primary components: insulin or insulin-like growth factor, dexamethasone (DXM), and 3-isobutyl-1-methylxanthine (IBMX).  In addition, rosiglitazone, a thiazolidinedione that binds to PPARγ and sensitizes fat cells to insulin, enhances adipogenesis.

During the differentiation process, 3T3-L1 cells undergo a post-confluent mitosis, which occurs 24 hours after induction with insulin, DXM, and IBMX, followed by growth arrest. After growth arrest occurs, the cells are committed to becoming adipocytes and express late markers of differentiation at day 3, after induction. Growth arrest of the cells is a requirement for terminal adipocyte differentiation. After 5-7 days post-induction, the cell morphology changes from the elongated, fibroblastic cells to rounded cells, and lipid droplets begin to accumulate.
 

General Literature References

The origin and definition of brite versus white and classical brown adipocytes: M. Rosenwald, et al.; Adipocyte 3, 4 (2014), Abstract; Full Text
Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution: V. Rizzatti, et al.; Eur. J. Histochem. 57, e24 (2013), Abstract; Full Text
Protocol for effective differentiation of 3T3-L1 cells to adipocytes: K. Zebisch, et al.; Anal. Biochem. 425, 88 (2012), Abstract;
Current methods of adipogenic differentiation of mesenchymal stem cells: M. A. Scott, et al.; Stem Cells Dev. 20, 1793 (2011), Abstract; Full Text
Rosiglitazone promotes development of a novel adipocyte population from bone marrow-derived circulating progenitor cells: J. T. Crossno, et al.; J. Clin. Invest. 116, 3220 (2006), Abstract; Full Text
Novel genes regulated by the insulin sensitizer rosiglitazone during adipocyte differentiation: T. Albrektsen, et al.; Diabetes 51, 1042 (2002), Abstract; Full Text
TNF-alpha inhibits 3T3-L1 adipocyte differentiation without downregulating the expression of C/EBPbeta and delta: S. Kurebayashi, et al.; Endocr. J. 48, 249 (2001), Abstract;
A synthetic antagonist for the peroxisome proliferator-activated receptor gamma inhibits adipocyte differentiation: H. M. Wright, et al.; J. Biol. Chem. 275, 1873 (2000), Abstract; Full Text
Adipocyte differentiation and gene expression: J. M. Ntambi, et al.; J. Nutr. 130, (2000), 3122S, Abstract; Full Text
CREB activation induces adipogenesis in 3T3-L1 cells: J. E. Reusch, et al.; Mol. Cell Biol. 20, 1008 (2000), Abstract; Full Text

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