Ultra-sensitive (7 pg/ml) AMP'D® HSP70 high sensitivity ELISA kit enabling the ability to use less sample and detect both baseline and upregulated levels of human, mouse and rat Hsp70 (Hsp72), a major chaperone, cancer biomarker, and key cell stress regulator.
The Hsp70 family of heat shock proteins contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.
Air pollution combined with high-fat feeding aggravates metabolic and cardiovascular diseases: A dangerous, oxidative, and immune-inflammatory association: L.C. Costa-Beber, et al.; Life Sci. 317, 121468 (2023), Abstract;
Oxidative and Cellular Stress Markers in Postmenopause Women with Diabetes: The Impact of Years of Menopause: C.F.V. Anklam, et al.; J. Diabetes Res. 2021, 3314871 (2021), Abstract;
Asthaxanthin improves aerobic exercise recovery without affecting heat tolerance in humans: C. Fleischmann, et al.; Front. Sports Act. Living 1, 17 (2019), Application(s): ELISA using human serum, Abstract; Full Text
Associations of inflammation with symptom burden in patients with acute myeloid leukemia: T.E. Lacourt, et al.; Psychoneuroendocrinology 89, 203 (2018), Application(s): ELISA using human plasma, Abstract;
Whole body precooling attenuates the extracellular HSP72, IL-6 and IL-10 responses after an acute bout of running in the heat: B.J. Lee, et al.; J. Sports Sci. 36, 414 (2018), Abstract;
Heat and hypoxic acclimation increase monocyte heat shock protein 72 but do not attenuate inflammation following hypoxic exercise: B.J. Lee, et al.; Front. Physiol. 8, 811 (2017), Application(s): ELISA using human plasma, Abstract; Full Text
Effect of Permissive Dehydration on Induction and Decay of Heat Acclimation, and Temperate Exercise Performance: R.A. Neal, et al.; Front. Physiol. 7, 564 (2016), Abstract; Full Text
A comparison of two commercially available ELISA methods for the quantification of human plasma heat shock protein 70 during rest and exercise stress: B.J. Lee, et al.; Cell Stress Chaperones 20, 917 (2015), Application(s): ELISA using human plasma, Abstract; Full Text
High-sensitivity ELISA kit for quantifying both normal and upregulated levels of Grp78/BiP for unfolded protein response, cancer and neurodegenerative disease research.
AMP'D® ELISA Signal Amplification Kit provides up to 50-fold increase in sensitivity over traditional ELISAs while detecting lower concentrations of target in samples.
