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AMP'D® ELISA Signal Amplification Kit

AMP'D® ELISA Signal Amplification Kit provides up to 50-fold increase in sensitivity over traditional ELISAs while detecting lower concentrations of target in samples.
 
ENZ-KIT-100-0001 96 wells 1 assay 82.00 USD
 
ENZ-KIT-100-0005 5x96 wells 5 assays 306.00 USD
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  • Designed to amplify sandwich/immunometric ELISA signal
  • Increase ELISA sensitivity greater than 10-fold
  • Flexibility to work with own targets
  • Convenient one or five 96-well plate formats for high throughput analysis
  • Easy-to-use, simple procedure with results in just 30 minutes
The AMP'D® ELISA Signal Amplification  kit is designed to replace traditional alkaline phosphatase (AP) substrates, such as pNPP (p-Nitrophenyl phosphate), with a combination substrate and amplifier system that results in greater sensitivity when compared to a classic substrate enzyme linked-immunosorbent assay (ELISA).



ENZ-KIT-100 plate
ENZ-KIT-100 kitbox

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ENZ-KIT-100 plate ENZ-KIT-100 kitbox

Product Specification

Sensitivity:Improves ELISA sensitivity ~10 to 50-fold.
 
Assay Time:30 minutes
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the amplification of ELISA sensitivity designed to replace traditional alkaline phosphatase (AP) substrates.

Assay should be read at a wavelength of 495nm.
 
Handling:Upon receipt, the kit should be stored at 4°C.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Signal Amplification Substrate, Amplifier, Substrate Diluent, and Amplifier Diluent
 
Scientific Background:In the AMP'D® ELISA Signal Amplification kit, bound AP converts a substrate that is utilized in a second enzyme reaction system which is initiated by addition of the amplifier reagent.  It is this amplification step that allows for greater (amplified) color production at lower analyte concentrations resulting in an increase in assay sensitivity.
 

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