AMP'D® ELISA Signal Amplification Kit provides up to 50-fold increase in sensitivity over traditional ELISAs while detecting lower concentrations of target in samples.
Designed to amplify sandwich/immunometric ELISA signal
Increase ELISA sensitivity greater than 10-fold
Flexibility to work with own targets
Convenient one or five 96-well plate formats for high throughput analysis
Easy-to-use, simple procedure with results in just 30 minutes
The AMP'D® ELISA Signal Amplification kit is designed to replace traditional alkaline phosphatase (AP) substrates, such as pNPP (p-Nitrophenyl phosphate), with a combination substrate and amplifier system that results in greater sensitivity when compared to a classic substrate enzyme linked-immunosorbent assay (ELISA).
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Product Details
Sensitivity:
Improves ELISA sensitivity ~10 to 50-fold.
Assay Time:
30 minutes
Applications:
ELISA, Colorimetric detection
Application Notes:
For the amplification of ELISA sensitivity designed to replace traditional alkaline phosphatase (AP) substrates.
Assay should be read at a wavelength of 495nm.
Wavelength:
495 nm
Handling:
Upon receipt, the kit should be stored at 4°C.
Shipping:
Blue Ice Not Frozen
Long Term Storage:
+4°C
Contents:
Signal Amplification Substrate, Amplifier, Substrate Diluent, and Amplifier Diluent
Scientific Background:
In the AMP'D® ELISA Signal Amplification kit, bound AP converts a substrate that is utilized in a second enzyme reaction system which is initiated by addition of the amplifier reagent. It is this amplification step that allows for greater (amplified) color production at lower analyte concentrations resulting in an increase in assay sensitivity.
Ultra-sensitive (7 pg/ml) AMP'D® HSP70 high sensitivity ELISA kit enabling the ability to use less sample and detect both baseline and upregulated levels of human, mouse and rat Hsp70 (Hsp72), a major chaperone, cancer biomarker, and key cell stress regulator.
