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AMPIPROBE® UP/Mg/Mh Extraction Controls

The AMPIPROBE® UP/Mg/Mh Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium and Mycoplasma hominis.
 
ENZ-GEN210-0500 500 µl Inquire for pricing
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  • Compatible witrh manual and automated DNA extraction methods
  • Compatible with most open qPCR platforms
The AMPIPROBE® UP/Mg/Mh Extraction Controls are designed to be used during the genomic DNA extraction of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium, and Mycoplasma hominis.
All performance specifications must be validated by end-user and will vary based on chosen extraction methods. Eluent from extraction can be used as extraction control for AMPIPROBE® UP/Mg/Mh Assay kit (Prod. No. ENZ-GEN209).
Plasmids in Extraction Controls are present at higher concentrations compared to assay controls included in the qPCR kit.  Extra care should be taken to avoid contamination.

Product Specification

Applications:qPCR
 
Application Notes:The AMPIPROBE® UP/Mg/Mh Extraction Controls is compatible with manual and automated DNA extraction methods as well as any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN QIAsymphony SP and Rotor-Gene Q.
 
Species reactivity:Ureaplasma spp., Mycoplasma genitalium, Mycoplasma hominis
 
Use/Stability:All components are stable at -20°C until the kit's expiration date.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Contents:Negative Extraction Control
UP/Mg/MH Positive Extraction Control
 
Scientific Background:Ureaplasma and Mycoplasma, which belong to the same Mycoplasmataceae family and Mollicutes class, are the smallest self-replicating organisms and are characterized by their lack of a cell wall. These characteristics and limited biosynthetic capabilities contribute to the parasitic nature of Ureaplasma and Mycoplasma.
The class of pathogens is present in healthy individuals but has been associated with many adverse conditions affecting the reproductive tract. Mycoplasma genitalium is associated with urethritis, cervical inflammation, and pelvic inflammatory disease. M. hominis is often present concurrently with Ureaplasma species and is associated with a variety of conditions ranging from pelvic inflammatory diseases, chorioamnionitis, postpartum endometritis bacterial vaginosis, arthritis, osteoarthritis, wound infections, and several conditions in neonates.
Ureaplasma and Mycoplasma, especially in combination with other conditions such as bacterial vaginosis or cervical incompetence, have been associated with adverse pregnancy outcomes, such as chorioamnionitis, spontaneous preterm labor and preterm premature rupture of membranes.
The AMPIPROBE® UMM Assay provides rapid and accurate results for the qualitative detection of Ureaplasma spp. (Ureaplasma parvum and Ureaplasma urealyticum), Mycoplasma genitalium, and Mycoplasma hominis DNA in a user-supplied sample of interest.
 
Protocol:SETUP of DNA Extraction control
The procedure described below is optimized for the QIAsymphony SP instrument (Qiagen) starting from an AMPTIMA® Multitest Swab Specimen collection tube.
Once thawed at room temperature, the AMPIPROBE Extraction Control tubes may be prepared. Label two 2.0 mL sample tubes with the appropriate control type (PC, NC), and then add 1.0 mL of blank APTIMA media to each tube.
Next, add 25 µL of each AMPIPROBE Extraction Control into their associated, labelled 2.0 mL tube. The extraction controls are now prepared and ready for use. Continue extraction procedure same as patient samples.

PCR SETUP
Please refer to the PCR SETUP protocol present in Product Manual AMPIPROBE® UP/Mg/Mh Assay Kit  (Catalog #: ENZ-GEN209-0100, 100 Assays) and treat UP/Mg/Mh Positive Extraction Control and Negative Extraction controls as Samples.
 
Note: The reagents required for the PCR Master Mix provided in the AMPIROBE® UP/Mg/Mh Assay kits will be sufficient to run also the UP/Mg/Mh Extraction Controls.
 
 

General Literature References

Ureaplasma urealyticum, Mycoplasma hominis and adverse pregnancy outcomes: R. Capoccia, et al.; Curr. Opin. Infect. Dis. 26, 231 (2013), Abstract;
Molecular Methods for the Detection of Mycoplasma and Ureaplasma Infections in Humans: K.B. Waites, et al.; J. Mol. Diagn. 5, 437 (2012), Abstract; Full Text
Mycoplasmas and ureaplasmas as neonatal pathogens: K.B. Waites, et al.; Clin. Microbiol. Rev. 18, 757 (2005), Abstract; Full Text
Mycoplasma genitalium: the aetiological agent of urethritis and other sexually transmitted diseases: J.S. Jensen; J. Eur. Acad. Dermatol. Venereol. 18, 1 (2004), Abstract;
Molecular Biology and Pathogenicity of Mycoplasmas: S. Razin, et al.; Microbiol. Mol. Biol. Rev. 4, 1094 (1998), Abstract; Full Text

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