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AMPIPROBE® NG/CT/TV Extraction Controls

The AMPIPROBE® NG/CT/TV Extraction Controls are designed to ensure an efficient genomic DNA extraction process and detection of Neisseria gonorrhoeae, Chlamydia trachomatis and Trichomonas vaginalis.
 
ENZ-GEN208-0500 500 µl Inquire for pricing
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  • Compatible with manual and automated DNA extraction methods
  • Compatible with most open qPCR platforms
The AMPIPROBE® NG/CT/TV Extraction Controls are designed to be used during the genomic DNA extraction of Neisseria gonorrhoeae, Chlamydia trachomatis and Trichomonas vaginalis.
All performance specifications must be validated by end-user and will vary based on chosen extraction methods. Eluent from extraction can be used as extraction control for AMPIPROBE® NG/CT/TV Assay kit (Prod. No. ENZ-GEN207).
Plasmids in Extraction Controls are present at higher concentrations compared to assay controls included in the qPCR kit. Extra care should be taken to avoid contamination.

Product Specification

Applications:qPCR
 
Application Notes:The AMPIPROBE® NG/CT/TV Extraction Controls is compatible with manual and automated DNA extraction methods as well as any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN QIAsymphony SP and Rotor-Gene Q.
 
Species reactivity:Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis
 
Use/Stability:All components are stable at -20°C until the kit's expiration date.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Contents:Negative Extraction Control
NG/CT/TV Positive Extraction Control
 
Scientific Background:Sexually transmitted infections caused by N. gonorrhoeae, C. trachomatis, and T. vaginalis DNA are among the most curable venereal diseases, but can all lead to serious conditions such as pelvic inflammatory disease (PID) if left untreated. Over 100 million new cases of gonorrhoeae each year are caused by Neisseria gonorrhoeae, a gram negative bacteria. Gonorrhoeae infections can lead to severe complications including epididymitis in men and PID in women. Consequently, PID can result in involuntary infertility and ectopic pregnancy. In addition, N. gonorrhoeae can infect the eyes of newborns during birth which can potentially lead to blindness.
Chlamydia trachomatis is also a gram negative bacterial pathogen that is the cause of sexually transmitted chlamydial infections with over 100 million cases each year. Infection with C. trachomatis can lead to PID, which consequently PID can result in ectopic pregnancy, infertility and chronic pelvic pain.
Trichomonas vaginalis is a parasite that infects over 270 million patients worldwide each year, making it the most common sexually transmitted disease (STD) agent in the world. Left untreated, a persistent T. vaginalis infection can lead to PID and adverse outcomes of pregnancy.
The current assay provides rapid and accurate results on the qualitative detection of N. gonorrhoeae, C. trachomatis, and T. vaginalis DNA in a user-supplied sample of interest.
 
Protocol:SETUP of DNA Extraction control
The procedure described below is optimized for the QIAsymphony SP instrument (Qiagen) starting from an AMPTIMA® Multitest Swab Specimen collection tube.
Once thawed at room temperature, the AMPIPROBE Extraction Control tubes may be prepared. Label two 2.0 mL sample tubes with the appropriate control type (PC, NC), and then add 1.0 mL of blank APTIMA media to each tube.
Next, add 25 µL of each AMPIPROBE Extraction Control into their associated, labelled 2.0 mL tube. The extraction controls are now prepared and ready for use. Continue extraction procedure same as patient samples.

PCR SETUP
Please refer to the PCR SETUP protocol present in Product Manual AMPIPROBE® NG/CT/TV Assay Kit (Catalog #: ENZ-GEN207-0100, 100 Assays) and treat NG/CT/TV Positive Extraction Control and Negative Extraction controls as Samples.

Note: The reagents required for the PCR Master Mix provided in the AMPIROBE® NG/CT/TV Assay kits will be sufficient to run also the NG/CT/TV Extraction Controls.
 

General Literature References

Global Prevalence And Incidence Of Selected Curable Sexually Transmitted Infections - 2008: World Health Organization; (2012), Full Text
Review and International Recommendation of Methods for Typing Neisseria gonorrhoeae Isolates and Their Implications for Improved Knowledge of Gonococcal Epidemiology, Treatment, and Biology : M. Unemo & J.A. Dillon; Clin. Microbiol. Rev. 3, 447 (2011), Abstract; Full Text
Global epidemiology and control of Trichomonas vaginalis : V.J. Johnston & D.C. Mabey; Curr. Opin. Infect. Dis. 21, 56 (2008), Abstract;
A review on infection with Chlamydia trachomatis: K Manavi; Best Pract. Res. Clin. Obstet. Gynaecol. 20, 941 (2006), Abstract;

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