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AMPIPROBE® NG/CT/TV Assay Kit

Sensitive and specific qPCR assay for the detection of NG/CT/TV
 
ENZ-GEN207-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 
  • Highly sensitive and specific assay
  • Low-cost alternative to other methods of NG/CT/TV detection
  • Compatible with most open qPCR platforms
  • Smaller sample input that allows remaining extracted samples to be used in other tests
The AMPIPROBE® NG/CT/TV Assay is a real-time polymerase chain reaction (qPCR) assay for the qualitative detection of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis genomic DNA. The kit uses the AMPIPROBE® assay platform which takes advantage of paired fluorophore- and quencher-labeled primers specific for each of the target species as well as an internal control. The kit contains all reagents necessary for PCR-based detection of N. gonorrhoeae, C. trachomatis, and T. vaginalis DNA. In addition, the assay kit includes a positive PCR control consisting of a mixture of the target templates and a negative PCR control that results negative for the targets, but positive for the internal control (human β-globin). Please read the complete kit insert before performing this assay.

Product Specification

Sensitivity:In a validation study approved by the New York State Department of Health, the AMPIPROBE® NG/CT/TV Assay was determined to have the following percentage of Sensitivity:

Neisseria gonorrhoeae - 100.00%
Chlamydia trachomatis - 100.00%
Trichomonas vaginalis - 90.60%
 
Applications:qPCR
 
Application Notes:The AMPIPROBE® NG/CT/TV Assay is compatible with any properly calibrated qPCR thermal cycler capable to detect fluorescence decay. It has been validated for use on the QIAGEN Rotor-Gene Q
 
Species reactivity:Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis
 
Specificity:In a validation study approved by the New York State Department of Health, the AMPIPROBE® NG/CT/TV Assay was determined to have the following percentage of Specificity:

Neisseria gonorrhoeae - 100.00%
Chlamydia trachomatis - 100.00%
Trichomonas vaginalis - 100.00%
 
Use/Stability:All components are stable at -20°C until the kit's expiration date.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Contents:AMPIGENE® HS Taq DNA Polymerase
AMPIGENE® dNTP Mix
AMPIPROBE® 5X Assay Buffer
AMPIPROBE® NG/CT/TV Primer Mix
NG/CT/TV Positive PCR Control
Negative PCR Control
Nuclease-free Water
 
Scientific Background:Sexually transmitted infections caused by N. gonorrhoeae, C. trachomatis, and T. vaginalis DNA are among the most curable venereal diseases, but can all lead to serious conditions such as pelvic inflammatory disease (PID) if left untreated. Over 100 million new cases of gonorrhoeae each year are caused by Neisseria gonorrhoeae, a gram negative bacteria. Gonorrhoeae infections can lead to severe complications including epididymitis in men and PID in women. Consequently, PID can result in involuntary infertility and ectopic pregnancy. In addition, N. gonorrhoeae can infect the eyes of newborns during birth which can potentially lead to blindness.
Chlamydia trachomatis is also a gram negative bacterial pathogen that is the cause of sexually transmitted chlamydial infections with over 100 million cases each year. Infection with C. trachomatis can lead to PID, which consequently PID can result in ectopic pregnancy, infertility and chronic pelvic pain.
Trichomonas vaginalis is a parasite that infects over 270 million patients worldwide each year, making it the most common sexually transmitted disease (STD) agent in the world. Left untreated, a persistent T. vaginalis infection can lead to PID and adverse outcomes of pregnancy.
The current assay provides rapid and accurate results on the qualitative detection of N.gonorrhoeae, C. trachomatis, and T. vaginalis DNA in a user-supplied sample of interest.
 

General Literature References

Global Prevalence And Incidence Of Selected Curable Sexually Transmitted Infections - 2008: World Health Organization; (2012), Full Text
Review and International Recommendation of Methods for Typing Neisseria gonorrhoeae Isolates and Their Implications for Improved Knowledge of Gonococcal Epidemiology, Treatment, and Biology : M. Unemo & J.A. Dillon; Clin. Microbiol. Rev. 3, 447 (2011), Abstract; Full Text
Global epidemiology and control of Trichomonas vaginalis : V.J. Johnston & D.C. Mabey; Curr. Opin. Infect. Dis. 21, 56 (2008), Abstract;
A review on infection with Chlamydia trachomatis: K Manavi; Best Pract. Res. Clin. Obstet. Gynaecol. 20, 941 (2006), Abstract;

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